Rapid indirect haemagglutination (rapid-IHA) using sensitized chick cells for serodiagnosis of amoebiasis at primary health centre level. 1989

S C Parija, and S Kasinathan, and R S Rao
Department of Microbiology, Fawaharlal Institute of Postgraduate Medical Education & Research, Pondicherry, India.

A successful modification of the indirect haemagglutination (IHA) test as a simple and rapid diagnostic procedure for serodiagnosis of amoebiasis at a primary health centre (PHC) level laboratory has been described in the present study. In this modified IHA test, the chick red blood cells (RBC), stabilized by the double aldehyde method, could be sensitized with amoebic antigen and then stored at 4 degrees C for up to 45 days for their subsequent use, as ready-made reagent directly in the IHA, without loss of sensitivity. The chick red cells settled quickly and their haemagglutination pattern could be determined within 30 to 45 min of incubation at room temperature with test sera. The IHA using double-aldehyde stabilized (DAS) chick cells, showed equal sensitivity and specificity with that of IHA using human O cells in diagnosing cases with different manifestations of amoebiasis. With the use of stored sensitized DAS chick cells directly in the IHA, the test could be performed and results obtained within 60 to 90 min of receipt of the sera to be tested. This makes IHA a simple and rapid procedure for its adaptation at a PHC level laboratory for the serodiagnosis of amoebiasis.

UI MeSH Term Description Entries
D011237 Predictive Value of Tests In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test. Negative Predictive Value,Positive Predictive Value,Predictive Value Of Test,Predictive Values Of Tests,Negative Predictive Values,Positive Predictive Values,Predictive Value, Negative,Predictive Value, Positive
D011320 Primary Health Care Care which provides integrated, accessible health care services by clinicians who are accountable for addressing a large majority of personal health care needs, developing a sustained partnership with patients, and practicing in the context of family and community. (JAMA 1995;273(3):192) Primary Care,Primary Healthcare,Care, Primary,Care, Primary Health,Health Care, Primary,Healthcare, Primary
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D006386 Hemagglutination Tests Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed) Hemagglutination Test,Test, Hemagglutination,Tests, Hemagglutination
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000562 Amebiasis Infection with any of various amebae. It is an asymptomatic carrier state in most individuals, but diseases ranging from chronic, mild diarrhea to fulminant dysentery may occur. Abscess, Amebic,Acanthamebiasis,Ameboma,Amoebiasis,Iodamoebiasis,Acanthamoeba Infection,Balamuthia Infection,Abscesses, Amebic,Acanthamebiases,Acanthamoeba Infections,Amebiases,Amebic Abscess,Amebic Abscesses,Amoebiases,Balamuthia Infections,Infection, Acanthamoeba,Infection, Balamuthia,Iodamoebiases
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

S C Parija, and S Kasinathan, and R S Rao
January 1989, Journal of hygiene, epidemiology, microbiology, and immunology,
S C Parija, and S Kasinathan, and R S Rao
November 1986, Journal of medical microbiology,
S C Parija, and S Kasinathan, and R S Rao
September 1974, The Indian journal of medical research,
S C Parija, and S Kasinathan, and R S Rao
April 1986, The Indian journal of medical research,
S C Parija, and S Kasinathan, and R S Rao
September 1969, Journal of clinical pathology,
S C Parija, and S Kasinathan, and R S Rao
July 1992, Zentralblatt fur Bakteriologie : international journal of medical microbiology,
S C Parija, and S Kasinathan, and R S Rao
August 2000, Journal of the Egyptian Society of Parasitology,
S C Parija, and S Kasinathan, and R S Rao
February 1992, The Journal of tropical medicine and hygiene,
S C Parija, and S Kasinathan, and R S Rao
July 1984, Journal of postgraduate medicine,
S C Parija, and S Kasinathan, and R S Rao
May 1977, Acta virologica,
Copied contents to your clipboard!