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A complex between the lac repressor headpiece and a fully symmetric tight-binding 22 bp lac operator was studied by 2D NMR. Several 2D NOE spectra were recorded for the complex in both H2O and 2H2O. Many NOE cross-peaks between the headpiece and DNA could be identified, and changes in the chemical shift of the DNA protons upon complex formation were analyzed. Comparison of these data with those obtained for a complex between the headpiece and a 14 bp half-operator, studied previously [Boelens, R., Scheek, R. M., Lamerichs, R. M. J. N., de Vlieg, J., van Boom, J. H., & Kaptein, R. (1987) in DNA-ligand interactions (Guschlbauer, W., & Saenger, W., Eds.) pp 191-215, Plenum, New York], shows that two headpieces form a specific complex with the 22 bp lac operator in which each headpiece binds in the same way as found for the 14 bp complex. The orientation of the recognition helix in the major groove of DNA in these complexes is opposite with respect to the dyad axis to that found for other repressors.
R M Lamerichs, and
R Boelens, and
G A van der Marel, and
J H van Boom, and
R Kaptein, and
F Buck, and
B Fera, and
H Rüterjans
January 1987,
Journal of molecular biology,
R M Lamerichs, and
R Boelens, and
G A van der Marel, and
J H van Boom, and
R Kaptein, and
F Buck, and
B Fera, and
H Rüterjans
July 1990,
Biochemistry,
R M Lamerichs, and
R Boelens, and
G A van der Marel, and
J H van Boom, and
R Kaptein, and
F Buck, and
B Fera, and
H Rüterjans
October 1988,
FEBS letters,
R M Lamerichs, and
R Boelens, and
G A van der Marel, and
J H van Boom, and
R Kaptein, and
F Buck, and
B Fera, and
H Rüterjans
May 1983,
European journal of biochemistry,
R M Lamerichs, and
R Boelens, and
G A van der Marel, and
J H van Boom, and
R Kaptein, and
F Buck, and
B Fera, and
H Rüterjans
January 1982,
The EMBO journal,
R M Lamerichs, and
R Boelens, and
G A van der Marel, and
J H van Boom, and
R Kaptein, and
F Buck, and
B Fera, and
H Rüterjans
January 1986,
Proceedings of the National Academy of Sciences of the United States of America,
R M Lamerichs, and
R Boelens, and
G A van der Marel, and
J H van Boom, and
R Kaptein, and
F Buck, and
B Fera, and
H Rüterjans
January 1982,
Proceedings of the National Academy of Sciences of the United States of America,
R M Lamerichs, and
R Boelens, and
G A van der Marel, and
J H van Boom, and
R Kaptein, and
F Buck, and
B Fera, and
H Rüterjans
February 1997,
Current opinion in structural biology,
R M Lamerichs, and
R Boelens, and
G A van der Marel, and
J H van Boom, and
R Kaptein, and
F Buck, and
B Fera, and
H Rüterjans
December 1999,
Structure (London, England : 1993),
R M Lamerichs, and
R Boelens, and
G A van der Marel, and
J H van Boom, and
R Kaptein, and
F Buck, and
B Fera, and
H Rüterjans
November 1983,
Proceedings of the National Academy of Sciences of the United States of America,
