-transparent.png){kind=logo}
The growth inhibitory activity in conditioned medium of African green monkey kidney epithelial (BSC-1) cells that has been shown to arise, at least in part, from transforming growth factor beta 2 (TGF-beta 2) [Hanks, S. K., Armour, R., Baldwin, J. H., Maldonado, F., Spiess, J., & Holley, R. W. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 79-82] was tested for growth inhibitory activity prior to and following acidification. Similar to TGF-beta 1 from human platelets, the inhibitory activity from BSC-1 cells demonstrated an 8-10-fold stimulation following acidification, showing that the activity was secreted from the cells in latent form. Conditioned medium from BSC-1 cells was collected, acidified, and fractionated by procedures that separate TGF-beta 1 and -2. Biological activity was assayed by using the BSC-1 cell proliferation assay. Two active proteins with properties similar to known TGF-beta 1 and TGF-beta 2 were identified. Identity was confirmed by using immunological and amino acid sequencing techniques. These results were consistent with Northern blot analysis of total BSC-1 RNA, using cDNA probes for TGF-beta 1 and TGF-beta 2, which demonstrated strong signals for both mRNAs. Metabolic labeling in conjunction with two-dimensional gel electrophoresis revealed that the cells secrete approximately 10% TGF-beta 1 and 90% TGF-beta 2.
J M McPherson, and
S J Sawamura, and
Y Ogawa, and
K Dineley, and
P Carrillo, and
K A Piez
August 1986,
Proceedings of the National Academy of Sciences of the United States of America,
J M McPherson, and
S J Sawamura, and
Y Ogawa, and
K Dineley, and
P Carrillo, and
K A Piez
November 1984,
Science (New York, N.Y.),
J M McPherson, and
S J Sawamura, and
Y Ogawa, and
K Dineley, and
P Carrillo, and
K A Piez
January 1987,
Methods in enzymology,
J M McPherson, and
S J Sawamura, and
Y Ogawa, and
K Dineley, and
P Carrillo, and
K A Piez
September 1983,
Proceedings of the National Academy of Sciences of the United States of America,
J M McPherson, and
S J Sawamura, and
Y Ogawa, and
K Dineley, and
P Carrillo, and
K A Piez
March 1972,
International journal of cancer,
J M McPherson, and
S J Sawamura, and
Y Ogawa, and
K Dineley, and
P Carrillo, and
K A Piez
September 1978,
Plasmid,
J M McPherson, and
S J Sawamura, and
Y Ogawa, and
K Dineley, and
P Carrillo, and
K A Piez
February 1971,
Biochimica et biophysica acta,
J M McPherson, and
S J Sawamura, and
Y Ogawa, and
K Dineley, and
P Carrillo, and
K A Piez
December 1989,
The Journal of cell biology,
J M McPherson, and
S J Sawamura, and
Y Ogawa, and
K Dineley, and
P Carrillo, and
K A Piez
February 1991,
Journal of neuroscience research,
J M McPherson, and
S J Sawamura, and
Y Ogawa, and
K Dineley, and
P Carrillo, and
K A Piez
January 1991,
The American journal of pathology,
