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In mammals, platelet activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a lipid mediator with biological activity at concentrations in the subnanomolar range. Although PAF is known to have many activities in mammals, little is known about its synthesis and importance in other vertebrate groups. We demonstrate here the synthesis of PAF from [3H]acetate by slices of trout gill, kidney, liver and spleen. PAF synthesis was stimulated by the calcium ionophore A23187 and was time-dependent. The radiolabeled PAF produced was characterized by TLC, HPLC, derivatization and by saponification and phospholipase A2 hydrolysis. These findings suggest that PAF may be an important mediator in fish.
M R Turner, and
R H Lumb
October 1988,
Immunology,
M R Turner, and
R H Lumb
January 1966,
Bulletin - Office international des epizooties,
M R Turner, and
R H Lumb
July 1983,
Revue canadienne de biologie experimentale,
M R Turner, and
R H Lumb
June 1980,
Bulletin of environmental contamination and toxicology,
M R Turner, and
R H Lumb
January 1985,
Zentralblatt fur Veterinarmedizin. Reihe A,
M R Turner, and
R H Lumb
January 1985,
Zentralblatt fur Veterinarmedizin. Reihe A,
M R Turner, and
R H Lumb
June 1983,
General and comparative endocrinology,
M R Turner, and
R H Lumb
January 1989,
Brain, behavior and evolution,
M R Turner, and
R H Lumb
October 1969,
Experientia,
M R Turner, and
R H Lumb
January 1988,
Comparative biochemistry and physiology. B, Comparative biochemistry,
