Periostin mediates cigarette smoke extract-induced proliferation and migration in pulmonary arterial smooth muscle cells. 2016

Xiao-Dong Wang, and Fang Li, and Dong-Bo Ma, and Xiang Deng, and Hui Zhang, and Jia Gao, and Li Hao, and Dan-Dan Liu, and Jing Wang
Department of Respiratory Disease, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Cigarette smoking is an important risk factor for pulmonary arterial hypertension (PAH). Pulmonary arterial smooth muscle cells (PASMCs) play a critical role in the pathogenesis of PAH-associated arterial remodeling. This study was done to explore the expression and biological roles of periostin in PASMCs following exposure to cigarette smoke extract (CSE). PASMCs were exposed to different concentrations of CSE and tested for gene expression and reactive oxygen species (ROS) production. PASMCs were incubated with recombinant periostin protein or transfected with small interfering RNA targeting periostin before CSE exposure and then examined for cell proliferation and migration. Compared to control cells, exposure to CSE led to a significant upregulation of periostin. Pretreatment with 5mM N-acetyl-l-cysteine (an inhibitor of ROS formation) or 10μM U0126 (an inhibitor of ERK1/2) significantly prevented the induction of periostin in CSE-treated PASMCs. The addition of recombinant periostin protein significantly enhanced the proliferation and migration of PASMCs. In contrast, knockdown of endogenous periostin counteracted the proliferation and migration of PASMCs induced by CSE treatment. In conclusion, CSE induces the expression of periostin in PASMCs via promotion of ROS and activation of ERK1/2. Periostin mediates the effects of CSE on PASMC proliferation and migration. These findings warrant further exploration of the roles of periostin in cigarette smoking-associated pulmonary arterial remodeling.

UI MeSH Term Description Entries
D011651 Pulmonary Artery The short wide vessel arising from the conus arteriosus of the right ventricle and conveying unaerated blood to the lungs. Arteries, Pulmonary,Artery, Pulmonary,Pulmonary Arteries
D002465 Cell Movement The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell. Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012907 Smoking Willful or deliberate act of inhaling and exhaling SMOKE from burning substances or agents held by hand. Smoking Behaviors,Smoking Habit,Behavior, Smoking,Behaviors, Smoking,Habit, Smoking,Habits, Smoking,Smoking Behavior,Smoking Habits
D015815 Cell Adhesion Molecules Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis. Cell Adhesion Molecule,Intercellular Adhesion Molecule,Intercellular Adhesion Molecules,Leukocyte Adhesion Molecule,Leukocyte Adhesion Molecules,Saccharide-Mediated Cell Adhesion Molecules,Saccharide Mediated Cell Adhesion Molecules,Adhesion Molecule, Cell,Adhesion Molecule, Intercellular,Adhesion Molecule, Leukocyte,Adhesion Molecules, Cell,Adhesion Molecules, Intercellular,Adhesion Molecules, Leukocyte,Molecule, Cell Adhesion,Molecule, Intercellular Adhesion,Molecule, Leukocyte Adhesion,Molecules, Cell Adhesion,Molecules, Intercellular Adhesion,Molecules, Leukocyte Adhesion
D015854 Up-Regulation A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins. Receptor Up-Regulation,Upregulation,Up-Regulation (Physiology),Up Regulation
D017382 Reactive Oxygen Species Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of SIGNAL TRANSDUCTION and GENE EXPRESSION, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS. Active Oxygen Species,Oxygen Radical,Oxygen Radicals,Pro-Oxidant,Reactive Oxygen Intermediates,Active Oxygen,Oxygen Species, Reactive,Pro-Oxidants,Oxygen, Active,Pro Oxidant,Pro Oxidants,Radical, Oxygen
D048049 Extracellular Signal-Regulated MAP Kinases A mitogen-activated protein kinase subfamily that is widely expressed and plays a role in regulation of MEIOSIS; MITOSIS; and post mitotic functions in differentiated cells. The extracellular signal regulated MAP kinases are regulated by a broad variety of CELL SURFACE RECEPTORS and can be activated by certain CARCINOGENS. ERK MAP Kinase,ERK MAP Kinases,Extracellular Signal-Regulated Kinase,Extracellular Signal-Regulated Kinases,Extracellular Signal-Regulated MAP Kinase,MAP Kinases, Extracellular Signal-Regulated,Extracellular Signal Regulated Kinase,Extracellular Signal Regulated Kinases,Extracellular Signal Regulated MAP Kinase,Extracellular Signal Regulated MAP Kinases,Kinase, ERK MAP,Kinase, Extracellular Signal-Regulated,Kinases, Extracellular Signal-Regulated,MAP Kinase, ERK,MAP Kinases, Extracellular Signal Regulated,Signal-Regulated Kinase, Extracellular
D049109 Cell Proliferation All of the processes involved in increasing CELL NUMBER including CELL DIVISION. Cell Growth in Number,Cellular Proliferation,Cell Multiplication,Cell Number Growth,Growth, Cell Number,Multiplication, Cell,Number Growth, Cell,Proliferation, Cell,Proliferation, Cellular
D055785 Gene Knockdown Techniques The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES. Gene Knock Down Techniques,Gene Knock Down,Gene Knock-Down,Gene Knock-Down Techniques,Gene Knockdown,Gene Knock Downs,Gene Knock-Down Technique,Gene Knock-Downs,Gene Knockdown Technique,Gene Knockdowns,Knock Down, Gene,Knock Downs, Gene,Knock-Down Technique, Gene,Knock-Down Techniques, Gene,Knock-Down, Gene,Knock-Downs, Gene,Knockdown Technique, Gene,Knockdown Techniques, Gene,Knockdown, Gene,Knockdowns, Gene,Technique, Gene Knock-Down,Technique, Gene Knockdown,Techniques, Gene Knock-Down,Techniques, Gene Knockdown

Related Publications

Xiao-Dong Wang, and Fang Li, and Dong-Bo Ma, and Xiang Deng, and Hui Zhang, and Jia Gao, and Li Hao, and Dan-Dan Liu, and Jing Wang
September 2015, Molecular medicine reports,
Xiao-Dong Wang, and Fang Li, and Dong-Bo Ma, and Xiang Deng, and Hui Zhang, and Jia Gao, and Li Hao, and Dan-Dan Liu, and Jing Wang
April 2010, Sheng li xue bao : [Acta physiologica Sinica],
Xiao-Dong Wang, and Fang Li, and Dong-Bo Ma, and Xiang Deng, and Hui Zhang, and Jia Gao, and Li Hao, and Dan-Dan Liu, and Jing Wang
August 2011, Journal of cellular biochemistry,
Xiao-Dong Wang, and Fang Li, and Dong-Bo Ma, and Xiang Deng, and Hui Zhang, and Jia Gao, and Li Hao, and Dan-Dan Liu, and Jing Wang
December 2011, The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology,
Xiao-Dong Wang, and Fang Li, and Dong-Bo Ma, and Xiang Deng, and Hui Zhang, and Jia Gao, and Li Hao, and Dan-Dan Liu, and Jing Wang
December 2020, Current medical science,
Xiao-Dong Wang, and Fang Li, and Dong-Bo Ma, and Xiang Deng, and Hui Zhang, and Jia Gao, and Li Hao, and Dan-Dan Liu, and Jing Wang
January 2021, Molecular medicine reports,
Xiao-Dong Wang, and Fang Li, and Dong-Bo Ma, and Xiang Deng, and Hui Zhang, and Jia Gao, and Li Hao, and Dan-Dan Liu, and Jing Wang
March 2019, Respiratory research,
Xiao-Dong Wang, and Fang Li, and Dong-Bo Ma, and Xiang Deng, and Hui Zhang, and Jia Gao, and Li Hao, and Dan-Dan Liu, and Jing Wang
March 2020, American journal of physiology. Heart and circulatory physiology,
Xiao-Dong Wang, and Fang Li, and Dong-Bo Ma, and Xiang Deng, and Hui Zhang, and Jia Gao, and Li Hao, and Dan-Dan Liu, and Jing Wang
January 2012, Journal of biomedicine & biotechnology,
Xiao-Dong Wang, and Fang Li, and Dong-Bo Ma, and Xiang Deng, and Hui Zhang, and Jia Gao, and Li Hao, and Dan-Dan Liu, and Jing Wang
January 2022, Evidence-based complementary and alternative medicine : eCAM,
Copied contents to your clipboard!