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We investigated whether X-irradiation could induce the enzyme superoxide dismutase (SOD) in intestinal muscle. Groups of rats received abdominal irradiation and the time course and dose response for SOD activity determined. Jejunal smooth muscle homogenates were analyzed for the activities of copper/zinc (CuZn) and manganese (Mn) SOD activity and for a mitochondrial marker enzyme, citrate synthase. A progressive rise in Mn SOD activity occurred at 20, 46, and 72 h after 1500 R. No significant changes in Cu-Zn SOD activity occurred at any time after 1500 R. At 20 h after 250 R of X-irradiation, Mn SOD activity increased but no further increase occurred at higher irradiation exposures. At the same time, CuZn SOD activity at 20 h after irradiation was greater than controls only at an exposure of 1000 R (p less than 0.05). Using Western blotting, we were able to clearly demonstrate an increase in immunoreactive Mn SOD protein in muscle samples 20 h after 1500 R. The rise in Mn SOD is not simply due to increase in mitochondrial numbers or increase in all mitochondrial enzyme activities because activity of the mitochondrial marker enzyme citrate synthase was decreased after X-irradiation. Transmission electron microscopic studies demonstrated damage to mitochondria after a dose of 3000 R. The data yield evidence that free radicals play a role in irradiation-induced intestinal smooth muscle injury.
R W Summers, and
B V Maves, and
R D Reeves, and
L J Arjes, and
L W Oberley
May 1997,
The American journal of physiology,
R W Summers, and
B V Maves, and
R D Reeves, and
L J Arjes, and
L W Oberley
December 1984,
Archives of biochemistry and biophysics,
R W Summers, and
B V Maves, and
R D Reeves, and
L J Arjes, and
L W Oberley
January 1999,
Toxicology letters,
R W Summers, and
B V Maves, and
R D Reeves, and
L J Arjes, and
L W Oberley
September 2004,
Biological & pharmaceutical bulletin,
R W Summers, and
B V Maves, and
R D Reeves, and
L J Arjes, and
L W Oberley
October 2013,
Current gene therapy,
R W Summers, and
B V Maves, and
R D Reeves, and
L J Arjes, and
L W Oberley
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R W Summers, and
B V Maves, and
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L J Arjes, and
L W Oberley
December 2005,
The European respiratory journal,
R W Summers, and
B V Maves, and
R D Reeves, and
L J Arjes, and
L W Oberley
April 1999,
Sheng li xue bao : [Acta physiologica Sinica],
R W Summers, and
B V Maves, and
R D Reeves, and
L J Arjes, and
L W Oberley
January 2001,
Stroke,
R W Summers, and
B V Maves, and
R D Reeves, and
L J Arjes, and
L W Oberley
November 1999,
Pharmacology,
