New SPE-LC-MS/MS method for simultaneous determination of multi-class cyanobacterial and algal toxins. 2017

Sevasti-Kiriaki Zervou, and Christophoros Christophoridis, and Triantafyllos Kaloudis, and Theodoros M Triantis, and Anastasia Hiskia
Institute of Nanoscience and Nanotechnology, National Center for Scientific Research "Demokritos", Patriarchou Grigoriou & Neapoleos, 15341 Athens, Greece.

Cyanobacterial and algal toxins comprise a large group of harmful metabolites, belonging to different chemical classes, with a variety of chemical structures, physicochemical properties and toxic activities. In this study, a fast, simple and sensitive analytical method was developed for the simultaneous determination of multi-class cyanobacterial and algal toxins in water. The target compounds were: Cylindrospermopsin, Anatoxin-a, Nodularin, 12 Microcystins ([D-Asp3]MC-RR, MC-RR, MC-YR, MC-HtyR, [D-Asp3]MC-LR, MC-LR, MC-HilR, MC-WR, MC-LA, MC-LY, MC-LW and MC-LF), Okadaic acid and Domoic acid. Analytes were determined using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A dual Solid Phase Extraction (SPE) cartridge assembly was applied for the extraction of target compounds from water. Optimized SPE parameters included cartridge material, initial sample pH, sequence of the cartridges in the SPE assembly as well as composition and volume of the elution solvent. The method was validated, providing acceptable mean recoveries and reproducibility for most analytes. Limits of detection were at the ngL-1 level. The method was successfully applied in real lake water samples from Greece, where a wide range of Microcystins were detected for the first time, at concentrations ranging from 0.034 to 63μgL-1.

UI MeSH Term Description Entries
D008387 Marine Toxins Toxic or poisonous substances elaborated by marine flora or fauna. They include also specific, characterized poisons or toxins for which there is no more specific heading, like those from poisonous FISHES. Marine Biotoxins,Phycotoxins
D002853 Chromatography, Liquid Chromatographic techniques in which the mobile phase is a liquid. Liquid Chromatography
D006115 Greece A country in southern Europe, bordering the Aegean Sea, Ionian Sea, and the Mediterranean Sea, between Albania and Turkey. The capital is Athens. Crete,Macedonia (Greece)
D000087522 Cyanobacteria Toxins Toxic secondary metabolites produced CYANOBACTERIA. Cyanobacterial Neurotoxin,Cyanobacterial Neurotoxins,Cyanobacterial Toxins,Cyanotoxins,Neurotoxin, Cyanobacterial
D001427 Bacterial Toxins Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases. Bacterial Toxin,Toxins, Bacterial,Toxin, Bacterial
D014874 Water Pollutants, Chemical Chemical compounds which pollute the water of rivers, streams, lakes, the sea, reservoirs, or other bodies of water. Chemical Water Pollutants,Landfill Leachate,Leachate, Landfill,Pollutants, Chemical Water
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D015394 Molecular Structure The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds. Structure, Molecular,Molecular Structures,Structures, Molecular
D052616 Solid Phase Extraction An extraction method that separates analytes using a solid phase and a liquid phase. It is used for preparative sample cleanup before analysis by CHROMATOGRAPHY and other analytical methods. Extraction, Solid Phase,Extractions, Solid Phase,Solid Phase Extractions
D052998 Microcystins Cyclic heptapeptides found in MICROCYSTIS and other CYANOBACTERIA. Hepatotoxic and carcinogenic effects have been noted. They are sometimes called cyanotoxins, which should not be confused with chemicals containing a cyano group (CN) which are toxic. Cyanoginosins

Related Publications

Sevasti-Kiriaki Zervou, and Christophoros Christophoridis, and Triantafyllos Kaloudis, and Theodoros M Triantis, and Anastasia Hiskia
January 2019, Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan,
Sevasti-Kiriaki Zervou, and Christophoros Christophoridis, and Triantafyllos Kaloudis, and Theodoros M Triantis, and Anastasia Hiskia
October 2018, Toxins,
Sevasti-Kiriaki Zervou, and Christophoros Christophoridis, and Triantafyllos Kaloudis, and Theodoros M Triantis, and Anastasia Hiskia
June 2019, Talanta,
Sevasti-Kiriaki Zervou, and Christophoros Christophoridis, and Triantafyllos Kaloudis, and Theodoros M Triantis, and Anastasia Hiskia
January 2024, Clinica chimica acta; international journal of clinical chemistry,
Sevasti-Kiriaki Zervou, and Christophoros Christophoridis, and Triantafyllos Kaloudis, and Theodoros M Triantis, and Anastasia Hiskia
October 2014, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Sevasti-Kiriaki Zervou, and Christophoros Christophoridis, and Triantafyllos Kaloudis, and Theodoros M Triantis, and Anastasia Hiskia
June 2020, Environmental research,
Sevasti-Kiriaki Zervou, and Christophoros Christophoridis, and Triantafyllos Kaloudis, and Theodoros M Triantis, and Anastasia Hiskia
January 2014, Chromatographia,
Sevasti-Kiriaki Zervou, and Christophoros Christophoridis, and Triantafyllos Kaloudis, and Theodoros M Triantis, and Anastasia Hiskia
March 2017, Food chemistry,
Sevasti-Kiriaki Zervou, and Christophoros Christophoridis, and Triantafyllos Kaloudis, and Theodoros M Triantis, and Anastasia Hiskia
January 2016, Analytical and bioanalytical chemistry,
Sevasti-Kiriaki Zervou, and Christophoros Christophoridis, and Triantafyllos Kaloudis, and Theodoros M Triantis, and Anastasia Hiskia
February 2022, Journal of AOAC International,
Copied contents to your clipboard!