Biomaterial Database

Methods for pH determination in human erythrocytes. 1989

D Büttner, and J Büttner

Institut für Klinische Chemie I, Medizinische Hochschule Hannover.

The methods described in the literature for pH determination in human erythrocytes, i.e. the direct potentiometric measurement of haemolysed erythrocytes and the 5,5-dimethyl-2,4-oxazolidinedione (DMO) method, were examined and compared. In spite of careful optimization of the experimental technique, a statistically significant difference of a few hundredths of a pH unit remained between the results of the two methods. The calculation of the cellular water space of the erythrocytes is suggested as a possible reason for this difference; in the DMO method this leads to uncertainty in the determination of the DMO concentration and therefore in the derived intracellular pH values. For clinical use, the direct pH measurement of haemolysed erythrocytes, using the technique described here, is recommended.

UI	MeSH Term	Description	Entries
D007202	Indicators and Reagents	Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)	Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D010080	Oxazoles	Five-membered heterocyclic ring structures containing an oxygen in the 1-position and a nitrogen in the 3-position, in distinction from ISOXAZOLES where they are at the 1,2 positions.	Oxazole,1,3-Oxazolium-5-Oxides,Munchnones,1,3 Oxazolium 5 Oxides
D011199	Potentiometry	Solution titration in which the end point is read from the electrode-potential variations with the concentrations of potential determining ions. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D004114	Dimethadione	An anticonvulsant that is the active metabolite of TRIMETHADIONE.	5,5-Dimethyl-2,4-oxazolidinedione,5,5-Dimethyloxazolidine-2,4-dione,Dimethyloxazolidinedione
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations