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The glucose-containing oligosaccharides formed by calf thyroid slices incubated with radioactive glucose were studied. A compound soluble in chloroform/methanol/water, 1:1:0.3 (vol/vol), was found that was indistinguishable from the previously described glucose-containing dolichyl diphosphate oligosaccharide formed by liver microsomes. Glycopeptides were prepared by treating the glycoproteins with pronase, the amino acids were removed with alkaline borohydride, and the products were examined by paper electrophoresis and chromatography. A saccharide equal to that which occurs in the glucose-containing dolichyl diphosphate oligosaccharide could not be detected but glucose was found in oligosaccharides that seemed to be smaller by about three to five monosaccharide residues. The same results were obtained by direct treatment of the glycoproteins with alkaline borohydride.
R J Staneloni, and
L F Leloir
October 1984,
Proceedings of the National Academy of Sciences of the United States of America,
R J Staneloni, and
L F Leloir
February 2000,
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R J Staneloni, and
L F Leloir
December 1978,
The Biochemical journal,
R J Staneloni, and
L F Leloir
March 1988,
Biochemistry,
R J Staneloni, and
L F Leloir
September 1984,
Biochemistry,
R J Staneloni, and
L F Leloir
January 2022,
Carbohydrate research,
R J Staneloni, and
L F Leloir
January 1950,
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R J Staneloni, and
L F Leloir
April 1981,
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R J Staneloni, and
L F Leloir
May 1969,
Biochemical and biophysical research communications,
R J Staneloni, and
L F Leloir
September 2018,
Carbohydrate research,
