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A new synthesis route for long phosphate-methylated oligodeoxynucleotides is described, which were used as antisense inhibitors of the DNA replication. Phosphate-methylated oligomers hybridize more strongly with natural DNA than their natural analogues, due to the absence of electrostatic interstrand repulsions. Compared with phosphate-ethylated and methyl phosphonate systems, phosphate-methylated systems are preferable as antisense DNA, which was concluded from the high Tm values and sharp melting transitions of duplexes of phosphate-methylated and natural DNA. By using the Sanger dideoxy technique, it was shown that a complementary phosphate-methylated 18-mer can effectively and site-specifically block the DNA replication process at room temperature.
H M Moody, and
M H van Genderen, and
L H Koole, and
H J Kocken, and
E M Meijer, and
H M Buck
October 2000,
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H M Moody, and
M H van Genderen, and
L H Koole, and
H J Kocken, and
E M Meijer, and
H M Buck
May 1991,
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H M Moody, and
M H van Genderen, and
L H Koole, and
H J Kocken, and
E M Meijer, and
H M Buck
October 1992,
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H M Moody, and
M H van Genderen, and
L H Koole, and
H J Kocken, and
E M Meijer, and
H M Buck
November 1994,
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H M Moody, and
M H van Genderen, and
L H Koole, and
H J Kocken, and
E M Meijer, and
H M Buck
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H M Moody, and
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July 1996,
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H M Moody, and
M H van Genderen, and
L H Koole, and
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August 1988,
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H M Moody, and
M H van Genderen, and
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H J Kocken, and
E M Meijer, and
H M Buck
January 1996,
Zhonghua yi xue za zhi,
H M Moody, and
M H van Genderen, and
L H Koole, and
H J Kocken, and
E M Meijer, and
H M Buck
February 1994,
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H M Moody, and
M H van Genderen, and
L H Koole, and
H J Kocken, and
E M Meijer, and
H M Buck
January 1991,
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