-transparent.png){kind=logo}
Some new relations between cytochrome P-450-dependent monooxygenases were discovered. Cytochrome b5, a representative of "microsomal" monooxygenases, was shown to form a highly specific complex with cytochrome P-450scc, a member of the "ferredoxin" monooxygenase family. This interaction is characterized by a dissociation constant, Kd, of 0.28 microM. The cytochrome P-450scc-cytochrome b5 complex may be cross-linked with water-soluble carbodiimide. Using proteolytic modification of cytochrome b5, it was shown that both hydrophilic and hydrophobic fragments of cytochrome b5 are involved in the interaction with cytochrome P-450scc. Cytochrome b5 immobilized via amino groups is an effective affinity matrix for cytochrome P-450scc purification. The role of some amino acid residues in cytochrome P-450scc interaction with cytochrome b5 was studied. The role and the nature of complexes in cytochrome P-450-dependent monooxygenases as well as interrelationships between "microsomal" and "ferredoxin" monooxygenases are discussed.
S A Usanov, and
V L Chashchin, and
A A Akhrem
June 1994,
FEBS letters,
S A Usanov, and
V L Chashchin, and
A A Akhrem
October 1981,
Archives of biochemistry and biophysics,
S A Usanov, and
V L Chashchin, and
A A Akhrem
December 1986,
Archives of biochemistry and biophysics,
S A Usanov, and
V L Chashchin, and
A A Akhrem
January 1985,
Drug metabolism and disposition: the biological fate of chemicals,
S A Usanov, and
V L Chashchin, and
A A Akhrem
June 1990,
Biokhimiia (Moscow, Russia),
S A Usanov, and
V L Chashchin, and
A A Akhrem
August 1988,
Biochemistry,
S A Usanov, and
V L Chashchin, and
A A Akhrem
January 1982,
Acta biologica et medica Germanica,
S A Usanov, and
V L Chashchin, and
A A Akhrem
April 1983,
Bioorganicheskaia khimiia,
S A Usanov, and
V L Chashchin, and
A A Akhrem
January 1984,
Drug metabolism and disposition: the biological fate of chemicals,
S A Usanov, and
V L Chashchin, and
A A Akhrem
September 1979,
The Journal of biological chemistry,
