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Eight electrophoretically homogeneous forms of cytochrome P-450 were isolated from liver microsomes of phenobarbital (PB)- and 3-methylcholanthrene (MC)-induced male Wistar rats, using chromatography on 1.8-diaminooctyl-Sepharose, SEAE-Sephacel and hydroxylapatite. These cytochrome forms were compared to those described in literature in terms of their ability to metabolize androstenedione (AD), benzphetamine (BP) and 7-ethoxyresorufin (7-ER). Cytochrome P-450b capable of catalyzing with a high specificity the 16-hydroxylation of AD and N-demethylation of BP, and cytochrome P-450e immunologically related to P-450b but incapable of catalyzing these reactions were isolated from PB-microsomes. Besides, a male-specific cytochrome P-450h catalyzing the 16 alpha-hydroxylation of AD was isolated from PB-microsomes. Cytochrome P-450c possessing a high 7-ER-O-deethylase activity, and a high spin cytochrome P-450d as well as cytochrome P-450a specifically catalyzing the 7 alpha-oxidation of AD were isolated from MC-microsomes. Two forms of cytochrome P-450 isolated from PB-microsomes possessed no such activities. Data from immunochemical analysis suggest that one of these forms can be identified as cytochrome P-450k. It is concluded that the specificity of metabolism and the molecular activity of Wistar rat liver cytochrome P-450 forms are comparable with the corresponding parameters of hemoproteins isolated from other rat species. At the same time, data from metabolic analysis are suggestive of differences in the levels of certain cytochrome P-450 forms, in particular P-450a.
L F Guliaeva, and
O G Khatsenko, and
K E Gerasimov, and
D V Mitrofanov, and
V M Mishin
July 1984,
Journal of biochemistry,
L F Guliaeva, and
O G Khatsenko, and
K E Gerasimov, and
D V Mitrofanov, and
V M Mishin
March 1984,
Journal of biochemistry,
L F Guliaeva, and
O G Khatsenko, and
K E Gerasimov, and
D V Mitrofanov, and
V M Mishin
March 1985,
Biokhimiia (Moscow, Russia),
L F Guliaeva, and
O G Khatsenko, and
K E Gerasimov, and
D V Mitrofanov, and
V M Mishin
August 1980,
Journal of biochemistry,
L F Guliaeva, and
O G Khatsenko, and
K E Gerasimov, and
D V Mitrofanov, and
V M Mishin
March 1986,
Journal of biochemistry,
L F Guliaeva, and
O G Khatsenko, and
K E Gerasimov, and
D V Mitrofanov, and
V M Mishin
August 1980,
Journal of biochemistry,
L F Guliaeva, and
O G Khatsenko, and
K E Gerasimov, and
D V Mitrofanov, and
V M Mishin
November 1985,
Biokhimiia (Moscow, Russia),
L F Guliaeva, and
O G Khatsenko, and
K E Gerasimov, and
D V Mitrofanov, and
V M Mishin
May 1984,
Biokhimiia (Moscow, Russia),
L F Guliaeva, and
O G Khatsenko, and
K E Gerasimov, and
D V Mitrofanov, and
V M Mishin
July 1982,
Biochemical and biophysical research communications,
L F Guliaeva, and
O G Khatsenko, and
K E Gerasimov, and
D V Mitrofanov, and
V M Mishin
February 1979,
The Journal of biological chemistry,
