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Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. 2016

Kevin P O'Rourke, and Sarah Ackerman, and Lukas E Dow, and Scott W Lowe
Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program, New York, USA; Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, USA.

In this protocol we describe our modifications to a method to isolate, culture and maintain mouse intestinal stem cells as crypt-villus forming organoids. These cells, isolated either from the small or large intestine, maintain self-renewal and multilineage differentiation potential over time. This provides investigators a tool to culture wild type or transformed intestinal epithelium, and a robust assay for stem cell tissue homeostasis in vitro.

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