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A modified horizontal capillary puller for fabrication of patch-clamp pipettes. 1989

G A Mealing, and J L Schwartz
Division of Biological Sciences, National Research Council, Ottawa, Ontario, Canada.

A simple low-cost modification to a conventional horizontal two-stage micropipette puller is described. It allows the production of pipettes suitable for patch-clamp studies. It requires that the hard-pull stage of the fabrication process be disabled and two heating cycles, each with a different heating filament temperature, be employed. The modification is described specifically for an Industrial Science Associates, Inc. M-1 micropipette puller. However, in principle it should be applicable to any horizontal two-stage puller using a solenoid to generate the pull force.

UI MeSH Term Description Entries
D004581 Electronics The study, control, and application of the conduction of ELECTRICITY through gases or vacuum, or through semiconducting or conducting materials. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Electronic
D004594 Electrophysiology The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.

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