-transparent.png){kind=logo}
As part of the strategy for screening for natural killer (NK) cell-specific monoclonal antibodies (MoAb) we have raised a number of murine NK-like cell lines in media containing interleukin-2 (IL-2). The detection of specific NK cell alloantigens on a C57BL/6 cell line in long-term culture in IL-2 is the subject of this paper. The C57BL/6 cell line has the morphology of large granular lymphocytes (LGL) and exhibits strong cytolytic activity against the archetype NK cell target, YAC-1. Absorption of three anti-NK antiserum, NZB anti-BALB/c (anti-NK-2.1), BALB/c anti-DBA/2 (anti-NK-3.1) and CE anti-CBA (anti-NK-4.1), with the C57BL/6 cell line removed the anti-NK activity from these antisera. Flow cytometric studies of the C57BL/6 cell line demonstrated significant binding of the anti-NK-1.1 MoAb produced by hybridoma PK136. Our results suggest that the C57BL/6 NK-like cell line exhibits some of the properties of naive NK cells and expresses all the known NK cell-specific alloantigens, NK-1.1, NK-2.1, NK-3.1 and NK-4.1 and therefore is potentially useful in selecting NK specific hybridomas and in studying the biology of NK cells.
Y C Smart, and
K L Stevenson, and
R F Thorne, and
W D Thomas, and
L H Hsu, and
R C Burton
January 1987,
Immunogenetics,
Y C Smart, and
K L Stevenson, and
R F Thorne, and
W D Thomas, and
L H Hsu, and
R C Burton
October 1989,
Immunology and cell biology,
Y C Smart, and
K L Stevenson, and
R F Thorne, and
W D Thomas, and
L H Hsu, and
R C Burton
February 1996,
Blood,
Y C Smart, and
K L Stevenson, and
R F Thorne, and
W D Thomas, and
L H Hsu, and
R C Burton
May 2010,
Zhonghua bing li xue za zhi = Chinese journal of pathology,
Y C Smart, and
K L Stevenson, and
R F Thorne, and
W D Thomas, and
L H Hsu, and
R C Burton
November 2002,
Leukemia research,
Y C Smart, and
K L Stevenson, and
R F Thorne, and
W D Thomas, and
L H Hsu, and
R C Burton
October 2021,
iScience,
Y C Smart, and
K L Stevenson, and
R F Thorne, and
W D Thomas, and
L H Hsu, and
R C Burton
February 2021,
Journal of fungi (Basel, Switzerland),
Y C Smart, and
K L Stevenson, and
R F Thorne, and
W D Thomas, and
L H Hsu, and
R C Burton
May 1981,
Journal of immunology (Baltimore, Md. : 1950),
Y C Smart, and
K L Stevenson, and
R F Thorne, and
W D Thomas, and
L H Hsu, and
R C Burton
February 2017,
Nihon rinsho. Japanese journal of clinical medicine,
Y C Smart, and
K L Stevenson, and
R F Thorne, and
W D Thomas, and
L H Hsu, and
R C Burton
June 1999,
Journal of hematotherapy,
