Use of an rRNA probe and restriction endonuclease analysis to fingerprint Pasteurella multocida isolated from turkeys and wildlife. 1989

K P Snipes, and D C Hirsh, and R W Kasten, and L M Hansen, and D W Hird, and T E Carpenter, and R H McCapes
Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis 95616.

Twenty-five isolates of the bacterium Pasteurella multocida were characterized (fingerprinted) phenotypically and genotypically in order to compare the abilities of various techniques to differentiate strains for epidemiologic studies of fowl cholera. Isolates were obtained over a 16-month period from turkeys dying from fowl cholera (six outbreak flocks) and from wildlife captured on premises with a history of the disease. The characteristics compared included (i) serotype, (ii) subspecies, (iii) antibiogram, (iv) presence of plasmid DNA, (v) restriction endonuclease analysis patterns of whole-cell DNA, and (vi) ribotype. Ribotyping, a method of highlighting DNA restriction site heterogeneity by using an rRNA probe, worked well for differentiating the strains of P. multocida when the majority of the other techniques could not. Ribotyping results correlated directly with and confirmed results obtained from restriction endonuclease analysis. Ribotyping demonstrated the presence of up to three strains of P. multocida in one outbreak flock, recurrence of a single strain in five of the flocks over an 11-month period, and the presence of common strains in turkeys and wildlife on the premises.

UI MeSH Term Description Entries
D008297 Male Males
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D010325 Pasteurella The oldest recognized genus of the family PASTEURELLACEAE. It consists of several species. Its organisms occur most frequently as coccobacillus or rod-shaped and are gram-negative, nonmotile, facultative anaerobes. Species of this genus are found in both animals and humans.
D010326 Pasteurella Infections Infections with bacteria of the genus PASTEURELLA. Pasteurellosis,Infections, Pasteurella,Infection, Pasteurella,Pasteurella Infection,Pasteurelloses
D010641 Phenotype The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment. Phenotypes
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D011201 Poultry Diseases Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild. Disease, Poultry,Diseases, Poultry,Poultry Disease
D004196 Disease Outbreaks Sudden increase in the incidence of a disease. The concept includes EPIDEMICS and PANDEMICS. Outbreaks,Infectious Disease Outbreaks,Disease Outbreak,Disease Outbreak, Infectious,Disease Outbreaks, Infectious,Infectious Disease Outbreak,Outbreak, Disease,Outbreak, Infectious Disease,Outbreaks, Disease,Outbreaks, Infectious Disease
D004197 Disease Reservoirs Animate or inanimate sources which normally harbor disease-causing organisms and thus serve as potential sources of disease outbreaks. Reservoirs are distinguished from vectors (DISEASE VECTORS) and carriers, which are agents of disease transmission rather than continuing sources of potential disease outbreaks. Humans may serve both as disease reservoirs and carriers. Disease Reservoir,Human Disease Reservoirs,Infectious Disease Reservoir,Reservoirs of Infection,Infectious Disease Reservoirs,Disease Reservoir, Human,Disease Reservoir, Infectious,Disease Reservoirs, Human,Human Disease Reservoir,Infection Reservoir,Infection Reservoirs,Reservoir, Disease,Reservoir, Infectious Disease,Reservoirs, Human Disease
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA

Related Publications

K P Snipes, and D C Hirsh, and R W Kasten, and L M Hansen, and D W Hird, and T E Carpenter, and R H McCapes
October 1990, Canadian journal of veterinary research = Revue canadienne de recherche veterinaire,
K P Snipes, and D C Hirsh, and R W Kasten, and L M Hansen, and D W Hird, and T E Carpenter, and R H McCapes
January 2002, Veterinary microbiology,
K P Snipes, and D C Hirsh, and R W Kasten, and L M Hansen, and D W Hird, and T E Carpenter, and R H McCapes
July 1985, American journal of veterinary research,
K P Snipes, and D C Hirsh, and R W Kasten, and L M Hansen, and D W Hird, and T E Carpenter, and R H McCapes
March 2004, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
K P Snipes, and D C Hirsh, and R W Kasten, and L M Hansen, and D W Hird, and T E Carpenter, and R H McCapes
January 1992, Avian diseases,
K P Snipes, and D C Hirsh, and R W Kasten, and L M Hansen, and D W Hird, and T E Carpenter, and R H McCapes
April 1992, Infection and immunity,
K P Snipes, and D C Hirsh, and R W Kasten, and L M Hansen, and D W Hird, and T E Carpenter, and R H McCapes
September 1978, Antimicrobial agents and chemotherapy,
K P Snipes, and D C Hirsh, and R W Kasten, and L M Hansen, and D W Hird, and T E Carpenter, and R H McCapes
January 1995, Avian diseases,
K P Snipes, and D C Hirsh, and R W Kasten, and L M Hansen, and D W Hird, and T E Carpenter, and R H McCapes
March 1999, Journal of medical microbiology,
K P Snipes, and D C Hirsh, and R W Kasten, and L M Hansen, and D W Hird, and T E Carpenter, and R H McCapes
September 1982, Canadian journal of microbiology,
Copied contents to your clipboard!