BIOMAT Database Logo BIOMAT Database Logo

Simultaneous immunoelectron microscopic visualization of protein B23 and C23 distribution in the HeLa cell nucleolus. 1989

M Biggiogera, and S Fakan, and S H Kaufmann, and A Black, and J H Shaper, and H Busch
Centre of Electron Microscopy, University of Lausanne, Switzerland.

The intranucleolar distribution of phosphoproteins B23 and C23 was visualized simultaneously by post-embedding immunoelectron microscopy in HeLa cell nucleoli, using specific antibodies. The data show that proteins B23 and C23 co-localize to the same nucleolar compartments, i.e., the dense fibrillar component and the granular component. Neither of the two antibodies is significantly associated with the fibrillar centers in these cells, although the fibrillar centers appear positive after silver staining. These findings suggest that other unidentified components must be responsible for the silver staining observed in the fibrillar centers of interphase nucleoli. The results are discussed in the light of previously reported data obtained by preembedding immunolabeling techniques and by silver staining, which both suggested a localization of protein C23 inside the fibrillar centers.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D009687 Nuclear Proteins Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. Nucleolar Protein,Nucleolar Proteins,Nuclear Protein,Protein, Nuclear,Protein, Nucleolar,Proteins, Nuclear,Proteins, Nucleolar
D010750 Phosphoproteins Phosphoprotein
D002466 Cell Nucleolus Within most types of eukaryotic CELL NUCLEUS, a distinct region, not delimited by a membrane, in which some species of rRNA (RNA, RIBOSOMAL) are synthesized and assembled into ribonucleoprotein subunits of ribosomes. In the nucleolus rRNA is transcribed from a nucleolar organizer, i.e., a group of tandemly repeated chromosomal genes which encode rRNA and which are transcribed by RNA polymerase I. (Singleton & Sainsbury, Dictionary of Microbiology & Molecular Biology, 2d ed) Plasmosome,Cell Nucleoli,Nucleoli, Cell,Nucleolus, Cell,Plasmosomes
D006367 HeLa Cells The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays. Cell, HeLa,Cells, HeLa,HeLa Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000090243 Nucleophosmin Predominantly localized in the CELL NUCLEOLUS, involved in diverse cellular processes such as ribosome biogenesis, cytoplasmic-nuclear trafficking, centrosome duplication, and regulation of p53. NO38 Protein,Nucleolar Phosphoprotein B23,Nucleolar Protein B23,Nucleophosmid,Numatrin,Phosphoprotein B23, Nucleolus,Protein B23,B23, Nucleolar Phosphoprotein,B23, Nucleolar Protein,B23, Nucleolus Phosphoprotein,B23, Protein,Nucleolus Phosphoprotein B23,Phosphoprotein B23, Nucleolar,Protein B23, Nucleolar,Protein, NO38
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal

Related Publications

M Biggiogera, and S Fakan, and S H Kaufmann, and A Black, and J H Shaper, and H Busch
Silver staining, immunofluorescence, and immunoelectron microscopic localization of nucleolar phosphoproteins B23 and C23.
January 1984, Chromosoma,
M Biggiogera, and S Fakan, and S H Kaufmann, and A Black, and J H Shaper, and H Busch
In vivo interaction of nucleophosmin/B23 and protein C23 during cell cycle progression in HeLa cells.
September 1999, Cancer letters,
M Biggiogera, and S Fakan, and S H Kaufmann, and A Black, and J H Shaper, and H Busch
[Isolation of the protein B23/nucleophosmin from HeLa cell nuclei].
January 2008, Prikladnaia biokhimiia i mikrobiologiia,
M Biggiogera, and S Fakan, and S H Kaufmann, and A Black, and J H Shaper, and H Busch
Immunoelectron microscopic characterization of nucleolus-associated domains during hibernation.
January 2011, Microscopy research and technique,
M Biggiogera, and S Fakan, and S H Kaufmann, and A Black, and J H Shaper, and H Busch
C23 interacts with B23, a putative nucleolar-localization-signal-binding protein.
April 1996, European journal of biochemistry,
M Biggiogera, and S Fakan, and S H Kaufmann, and A Black, and J H Shaper, and H Busch
[Difference in the distribution and content of nucleolar protein B23 between interphase and mitotic HeLa cells].
June 1994, Shi yan sheng wu xue bao,
M Biggiogera, and S Fakan, and S H Kaufmann, and A Black, and J H Shaper, and H Busch
Immunoelectron microscopic visualization of the gap junction protein connexin 40 in the mammalian heart.
January 1993, European journal of morphology,
M Biggiogera, and S Fakan, and S H Kaufmann, and A Black, and J H Shaper, and H Busch
Localization of nucleolar phosphoproteins B23 and C23 during mitosis.
June 1983, Experimental cell research,
M Biggiogera, and S Fakan, and S H Kaufmann, and A Black, and J H Shaper, and H Busch
State of oncomarker protein B23/nucleophosmin in HeLa cells.
July 2010, Biochemistry. Biokhimiia,
M Biggiogera, and S Fakan, and S H Kaufmann, and A Black, and J H Shaper, and H Busch
Proteins C23 and B23 are the major nucleolar silver staining proteins.
August 1979, Life sciences,
Copied contents to your clipboard!