Biomaterial Database

Model calculations of oxygen supply to tissue slice preparations. 1989

H G Lipinski

Department of Neurology and Clinical Neurophysiology, Technical University Munich, Federal Republic of Germany.

Excised tissue slice preparations are widely used in experimental medicine, pharmacology and physiology. Since slices are separated from the vascular system they have to be supplied with oxygen from the bath solution in which the slices are fixed. Otto Warburg designed a simple model of oxygen diffusion in such a tissue preparation. His model does not utilise some important parameters which may influence the oxygen distribution in the tissue: Unstirred bathing, non-vital superficial layers of tissue slices, damage by the cutting procedure and the influence of volume fraction and tortuosity over the oxygen supply. A compartment model has been designed to test how these parameters affect the oxygen distribution in tissue slices. The calculations have shown that all parameters may considerably affect the oxygen supply to tissue slices. Therefore, they have to be considered in the analysis of oxygen distribution and consumption in tissue slice preparations.

UI	MeSH Term	Description	Entries
D008433	Mathematics	The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Mathematic
D008954	Models, Biological	Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.	Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D010101	Oxygen Consumption	The rate at which oxygen is used by a tissue; microliters of oxygen STPD used per milligram of tissue per hour; the rate at which oxygen enters the blood from alveolar gas, equal in the steady state to the consumption of oxygen by tissue metabolism throughout the body. (Stedman, 25th ed, p346)	Consumption, Oxygen,Consumptions, Oxygen,Oxygen Consumptions
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D046508	Culture Techniques	Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.	Culture Technique,Technique, Culture,Techniques, Culture

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