Retinoylation (covalent modification by retinoic acid) of Rho-GDIβ in the human myeloid leukemia cell line HL60 and its functional significance. 2016

Noriko Takahashi, and Toshihiro Ohba, and Masahiko Imai, and Shinya Hasegawa, and Katsuhiko Takahashi, and Masahiro Yamasaki, and Yuri Kameoka
Laboratory of Physiological Chemistry, Institute of Medicinal Chemistry, Hoshi University, Shinagawa, Tokyo 142-8501, Japan. Electronic address: t-noriko@hoshi.ac.jp.

Retinoic acid (RA) has a variety of biological effects in mammalian cells and tissues. It is well known that RA induces differentiation of human acute promyelocytic leukemia (APL) HL60 cells, fresh human APL cells, and clinical remission in patients with APL. Retinoylation (acylation of proteins by RA) is a possible pathway for RA action. However, an understanding of the role that retinoylation plays in the actions of RA is lacking. In the current study, several retinoylated proteins were detected in RA-treated HL60 fractions following Mono Q anion exchange chromatography and analysis using two-dimensional polyacrylamide gel electrophoresis. One of the retinoylated proteins was identified as Rho-GDIβ (28kDa) by TOF-MS and co-migration with Rho-GDIβ (28kDa). Truncated Rho-GDIβ (23kDa, N∆19), a product of cleavage by caspase-3, was not retinoylated. RA covalently bound to the Thr2 residue in Rho-GDIβ (5kDa), which is the second product resulting from the cleavage of Rho-GDIβ (28kDa) by caspase-3. RA treatment increased the level of Rho-GDIβ (28kDa) and decreased the level of Rho-GDIβ (23kDa). RA did not induce caspase-3 activity or Rho-GDIβ mRNA expression. It is likely that retinoylation of Rho-GDIβ increases its metabolic stability.

UI MeSH Term Description Entries
D007951 Leukemia, Myeloid Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites. Granulocytic Leukemia,Leukemia, Granulocytic,Leukemia, Myelocytic,Leukemia, Myelogenous,Myelocytic Leukemia,Myelogenous Leukemia,Myeloid Leukemia,Leukemia, Monocytic, Chronic,Monocytic Leukemia, Chronic,Chronic Monocytic Leukemia,Chronic Monocytic Leukemias,Granulocytic Leukemias,Leukemia, Chronic Monocytic,Leukemias, Chronic Monocytic,Leukemias, Granulocytic,Leukemias, Myelocytic,Leukemias, Myelogenous,Leukemias, Myeloid,Monocytic Leukemias, Chronic,Myelocytic Leukemias,Myelogenous Leukemias,Myeloid Leukemias
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000215 Acylation The addition of an organic acid radical into a molecule.
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D012313 RNA A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed) RNA, Non-Polyadenylated,Ribonucleic Acid,Gene Products, RNA,Non-Polyadenylated RNA,Acid, Ribonucleic,Non Polyadenylated RNA,RNA Gene Products,RNA, Non Polyadenylated
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D014212 Tretinoin An important regulator of GENE EXPRESSION during growth and development, and in NEOPLASMS. Tretinoin, also known as retinoic acid and derived from maternal VITAMIN A, is essential for normal GROWTH; and EMBRYONIC DEVELOPMENT. An excess of tretinoin can be teratogenic. It is used in the treatment of PSORIASIS; ACNE VULGARIS; and several other SKIN DISEASES. It has also been approved for use in promyelocytic leukemia (LEUKEMIA, PROMYELOCYTIC, ACUTE). Retinoic Acid,Vitamin A Acid,Retin-A,Tretinoin Potassium Salt,Tretinoin Sodium Salt,Tretinoin Zinc Salt,Vesanoid,all-trans-Retinoic Acid,beta-all-trans-Retinoic Acid,trans-Retinoic Acid,Acid, Retinoic,Acid, Vitamin A,Acid, all-trans-Retinoic,Acid, beta-all-trans-Retinoic,Acid, trans-Retinoic,Potassium Salt, Tretinoin,Retin A,Salt, Tretinoin Potassium,Salt, Tretinoin Sodium,Salt, Tretinoin Zinc,Sodium Salt, Tretinoin,Zinc Salt, Tretinoin,all trans Retinoic Acid,beta all trans Retinoic Acid,trans Retinoic Acid
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines
D053148 Caspase 3 A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA. CASP3,Apopain,Caspase-3,Pro-Caspase-3,Procaspase-3,Pro Caspase 3,Procaspase 3

Related Publications

Noriko Takahashi, and Toshihiro Ohba, and Masahiko Imai, and Shinya Hasegawa, and Katsuhiko Takahashi, and Masahiro Yamasaki, and Yuri Kameoka
March 1989, The Journal of biological chemistry,
Noriko Takahashi, and Toshihiro Ohba, and Masahiko Imai, and Shinya Hasegawa, and Katsuhiko Takahashi, and Masahiro Yamasaki, and Yuri Kameoka
February 1994, The Journal of biological chemistry,
Noriko Takahashi, and Toshihiro Ohba, and Masahiko Imai, and Shinya Hasegawa, and Katsuhiko Takahashi, and Masahiro Yamasaki, and Yuri Kameoka
January 1990, Methods in enzymology,
Noriko Takahashi, and Toshihiro Ohba, and Masahiko Imai, and Shinya Hasegawa, and Katsuhiko Takahashi, and Masahiro Yamasaki, and Yuri Kameoka
August 1994, European journal of haematology,
Noriko Takahashi, and Toshihiro Ohba, and Masahiko Imai, and Shinya Hasegawa, and Katsuhiko Takahashi, and Masahiro Yamasaki, and Yuri Kameoka
October 1990, Cancer research,
Noriko Takahashi, and Toshihiro Ohba, and Masahiko Imai, and Shinya Hasegawa, and Katsuhiko Takahashi, and Masahiro Yamasaki, and Yuri Kameoka
January 1990, Neoplasma,
Noriko Takahashi, and Toshihiro Ohba, and Masahiko Imai, and Shinya Hasegawa, and Katsuhiko Takahashi, and Masahiro Yamasaki, and Yuri Kameoka
March 1997, Biochemical and biophysical research communications,
Noriko Takahashi, and Toshihiro Ohba, and Masahiko Imai, and Shinya Hasegawa, and Katsuhiko Takahashi, and Masahiro Yamasaki, and Yuri Kameoka
January 1994, Blood,
Noriko Takahashi, and Toshihiro Ohba, and Masahiko Imai, and Shinya Hasegawa, and Katsuhiko Takahashi, and Masahiro Yamasaki, and Yuri Kameoka
July 2008, Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi,
Noriko Takahashi, and Toshihiro Ohba, and Masahiko Imai, and Shinya Hasegawa, and Katsuhiko Takahashi, and Masahiro Yamasaki, and Yuri Kameoka
September 2006, Zhonghua yi xue za zhi,
Copied contents to your clipboard!