It has been suggested that gloved hands could be washed between patient treatments in the dental surgery and gloves re-used, provided they are undamaged. A series of experiments are described, which evaluate the effectiveness of four handwashing agents at removing defined bacterial inoculae from two types of latex rubber glove, a dedicated dental procedure glove (Regent Biogel D) which has a rough surface and a smooth-surfaced examination glove (Microtouch). The four agents tested were povidone iodine (Betadine), chlorhexidine (Hibiscrub), 60% iso-propyl alcohol, and a detergent triclosan preparation (Kleenex washcream). The Biogel D gloves required slightly shorter washing times to eradicate organisms than the Microtouch gloves. It was found that washing with water alone reduced the organisms on gloves by 300-1000-fold, but a minimum washing time of 20 seconds with povidone iodine or chlorhexidine was required to eradicate all the organisms inoculated, except bacterial spores, from both glove surfaces. Povidone-iodine and chlorhexidine were more effective washing agents than iso-propyl alcohol and triclosan soap.