-transparent.png){kind=logo}
We describe a microassay for measuring galactose (Gal) and galactose 1-phosphate (Gal-1-P) in dried blood spots. After a coupled enzyme reaction involving galactose dehydrogenase (GADH, EC 1.1.1.48) and alkaline phosphatase (AP, EC 3.1.3.1) in a microplate well, NADH fluorescence is measured by a highly sensitive fluorometric microplate reader, capable of rapid measurement of fluorescence (2 min per 96 samples). Within- and between-run CVs for measurements of Gal at 90 mg/L with Gal-1-P at 130 mg/L were both less than 5% (n = 8), and analytical recoveries for Gal at 90 mg/L and Gal-1-P at 130 mg/L were 98% and 92%, respectively. Five hundred dried blood-spot samples can be assayed within 2 h, with full calculation of results by an on-line microcomputer. This rapid and reliable assay system is very useful for the routine screening of newborns for galactosemia.
A Yamaguchi, and
M Fukushi, and
Y Mizushima, and
Y Shimizu, and
N Takasugi, and
S Arashima, and
K Ohyanagi
June 2000,
Clinical chemistry,
A Yamaguchi, and
M Fukushi, and
Y Mizushima, and
Y Shimizu, and
N Takasugi, and
S Arashima, and
K Ohyanagi
November 1992,
Clinica chimica acta; international journal of clinical chemistry,
A Yamaguchi, and
M Fukushi, and
Y Mizushima, and
Y Shimizu, and
N Takasugi, and
S Arashima, and
K Ohyanagi
July 1994,
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies,
A Yamaguchi, and
M Fukushi, and
Y Mizushima, and
Y Shimizu, and
N Takasugi, and
S Arashima, and
K Ohyanagi
July 1988,
Medical laboratory sciences,
A Yamaguchi, and
M Fukushi, and
Y Mizushima, and
Y Shimizu, and
N Takasugi, and
S Arashima, and
K Ohyanagi
March 1991,
The Journal of the Oklahoma State Medical Association,
A Yamaguchi, and
M Fukushi, and
Y Mizushima, and
Y Shimizu, and
N Takasugi, and
S Arashima, and
K Ohyanagi
October 1969,
Clinical chemistry,
A Yamaguchi, and
M Fukushi, and
Y Mizushima, and
Y Shimizu, and
N Takasugi, and
S Arashima, and
K Ohyanagi
October 1993,
Analytical biochemistry,
A Yamaguchi, and
M Fukushi, and
Y Mizushima, and
Y Shimizu, and
N Takasugi, and
S Arashima, and
K Ohyanagi
April 1971,
The New England journal of medicine,
A Yamaguchi, and
M Fukushi, and
Y Mizushima, and
Y Shimizu, and
N Takasugi, and
S Arashima, and
K Ohyanagi
October 1989,
Clinical chemistry,
A Yamaguchi, and
M Fukushi, and
Y Mizushima, and
Y Shimizu, and
N Takasugi, and
S Arashima, and
K Ohyanagi
February 2019,
Luminescence : the journal of biological and chemical luminescence,
