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A method has been described for the detection and estimation of 3,4-dehydroproline using initial oxidation with isatin or H2O2 and subsequent reaction with p-dimethylamino benzaldehyde (Ehrlich reagent). The method is sensitive enough to detect as low as 0.6 micrograms of 3,4-dehydroproline/sq. cm on paper chromatogram. 3,4-dehydroproline could also be estimated by the classical H2O2-oxidation procedure employed for hydroxyproline apparently yielding the same chromophore on a molar basis. However, when estimated by the chloramine-T oxidation method, its sensitivity was only 1/100th of that of 4-hydroxyproline. The usefulness of these procedures in the detection and estimation of 3,4-dehydroproline has been described.
B Panikkar, and
R Kuttan
October 1962,
Archives of biochemistry and biophysics,
B Panikkar, and
R Kuttan
November 1973,
Toxicology and applied pharmacology,
B Panikkar, and
R Kuttan
October 1979,
Archivos de biologia y medicina experimentales,
B Panikkar, and
R Kuttan
October 1983,
Plant physiology,
B Panikkar, and
R Kuttan
January 2007,
Journal of combinatorial chemistry,
B Panikkar, and
R Kuttan
October 1977,
Archives of biochemistry and biophysics,
B Panikkar, and
R Kuttan
January 2009,
Biopolymers,
B Panikkar, and
R Kuttan
April 1979,
Research communications in chemical pathology and pharmacology,
B Panikkar, and
R Kuttan
June 1994,
Carbohydrate research,
