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Realization of 1D 1H spectra of superfused brain slices of rats is described. Two-dimensional 1H-1H COSY spectra have been used for the direct assignment of resonances on living tissue (lactate, GABA, N-acetyl aspartate, aspartate, glutamine/glutamate, creatine/phosphocreatine, taurine, inositol, choline/ethanolamine). Utilization of the SUPERCOSY sequence permits simultaneously the enhancement of off-diagonal signals for small metabolites and the reduction of the signals for water and macromolecules.
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