A Versatile Microarray Immobilization Strategy Based on a Biorthogonal Reaction Between Tetrazine and Trans-Cyclooctene. 2017

Ping Wang, and Liqian Gao, and Haipeng Lei, and Su Seong Lee, and Shao Q Yao, and Hongyan Sun
Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China.

Given its increasing importance in transforming biomedical research in recent years, microarray technology has become highly popular as a powerful screening platform in detecting biomolecule interactions, discovering new inhibitors, and identifying biomarkers as well as diagnosing disease. The success of microarray technology in various biological applications is highly dependent on the accessibility, the functionality, and the density of the surface bound biomolecules. Therefore, compound immobilization represents a critical step for the successful implementation of microarray screening. Herein we describe a fast and site-specific microarray immobilization approach by using trans-cyclooctene-tetrazine ligation. This approach not only ensures fast immobilization and uniform display of biomolecules, but also allows the optimum orientation of biomolecules after immobilization. All these excellent properties facilitate subsequent interactions of the biomolecules and their interacting partners during the screening process. We envision that the immobilization strategy described here can find useful applications in many other microarray related studies.

UI MeSH Term Description Entries
D009682 Magnetic Resonance Spectroscopy Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING). In Vivo NMR Spectroscopy,MR Spectroscopy,Magnetic Resonance,NMR Spectroscopy,NMR Spectroscopy, In Vivo,Nuclear Magnetic Resonance,Spectroscopy, Magnetic Resonance,Spectroscopy, NMR,Spectroscopy, Nuclear Magnetic Resonance,Magnetic Resonance Spectroscopies,Magnetic Resonance, Nuclear,NMR Spectroscopies,Resonance Spectroscopy, Magnetic,Resonance, Magnetic,Resonance, Nuclear Magnetic,Spectroscopies, NMR,Spectroscopy, MR
D010455 Peptides Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS. Peptide,Polypeptide,Polypeptides
D006573 Heterocyclic Compounds, 1-Ring Organic compounds that contain a ring structure made up of carbon and one or more additional elements such as nitrogen and oxygen. Heterocyclic Cpds, 1-Ring,1-Ring Heterocyclic Compounds,1-Ring Heterocyclic Cpds,Heterocyclic Compounds, 1 Ring,Heterocyclic Cpds, 1 Ring
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D046228 Microarray Analysis The simultaneous analysis, on a microchip, of multiple samples or targets arranged in an array format. Microarray Analytical Devices,Microarray Microchips,Nanoarray Analytical Devices,Analysis, Microarray,Analytical Device, Microarray,Analytical Device, Nanoarray,Analytical Devices, Microarray,Analytical Devices, Nanoarray,Device, Microarray Analytical,Device, Nanoarray Analytical,Devices, Microarray Analytical,Devices, Nanoarray Analytical,Microarray Analytical Device,Microarray Microchip,Microchip, Microarray,Microchips, Microarray,Nanoarray Analytical Device
D049452 Green Fluorescent Proteins Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH. Green Fluorescent Protein,Green-Fluorescent Protein,Green-Fluorescent Proteins,Fluorescent Protein, Green,Fluorescent Proteins, Green,Protein, Green Fluorescent,Protein, Green-Fluorescent,Proteins, Green Fluorescent,Proteins, Green-Fluorescent
D019817 Benzoic Acid A fungistatic compound that is widely used as a food preservative. It is conjugated to GLYCINE in the liver and excreted as hippuric acid. Potassium Benzoate,Ucephan,Acid, Benzoic,Benzoate, Potassium
D034242 Cyclooctanes A group of compounds with an 8-carbon ring. They may be saturated or unsaturated. Benzocyclooctanes,Benzocyclooctenes,Cyclooctenes,Cyclooctodienes,Dibenzocyclooctanes

Related Publications

Ping Wang, and Liqian Gao, and Haipeng Lei, and Su Seong Lee, and Shao Q Yao, and Hongyan Sun
October 2014, Chemical communications (Cambridge, England),
Ping Wang, and Liqian Gao, and Haipeng Lei, and Su Seong Lee, and Shao Q Yao, and Hongyan Sun
December 2022, Biophysical reports,
Ping Wang, and Liqian Gao, and Haipeng Lei, and Su Seong Lee, and Shao Q Yao, and Hongyan Sun
January 2017, Angewandte Chemie (International ed. in English),
Ping Wang, and Liqian Gao, and Haipeng Lei, and Su Seong Lee, and Shao Q Yao, and Hongyan Sun
October 2021, Bioorganic & medicinal chemistry,
Ping Wang, and Liqian Gao, and Haipeng Lei, and Su Seong Lee, and Shao Q Yao, and Hongyan Sun
May 2013, Chemical communications (Cambridge, England),
Ping Wang, and Liqian Gao, and Haipeng Lei, and Su Seong Lee, and Shao Q Yao, and Hongyan Sun
June 2020, Journal of the American Chemical Society,
Ping Wang, and Liqian Gao, and Haipeng Lei, and Su Seong Lee, and Shao Q Yao, and Hongyan Sun
October 2022, Chembiochem : a European journal of chemical biology,
Ping Wang, and Liqian Gao, and Haipeng Lei, and Su Seong Lee, and Shao Q Yao, and Hongyan Sun
July 2014, Chemical communications (Cambridge, England),
Ping Wang, and Liqian Gao, and Haipeng Lei, and Su Seong Lee, and Shao Q Yao, and Hongyan Sun
January 2016, Macromolecules,
Ping Wang, and Liqian Gao, and Haipeng Lei, and Su Seong Lee, and Shao Q Yao, and Hongyan Sun
January 2020, ACS nano,
Copied contents to your clipboard!