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Mouse interleukin 4 (IL-4) has been shown to act on B cells as an induction factor for Ig class switch. We studied the characteristics of IL-4-regulated Ig isotype production in lipopolysaccharide (LPS)-stimulated splenic B-cell cultures with emphasis on the comparison between the IgG1 and IgE responses. The results show that the kinetics for the appearance of IgG1 and IgE isotypes are similar, but that the dose of IL-4 required for the induction of an IgE response is 3-10 times higher than that for an IgG1 response. No requirement for T cells was found for the induction of either isotype. Pre-incubation of cells for 24 h with IL-4 alone was sufficient to induce an IgG1 response when cells were recultured with LPS from days 1 to 6. However, the simultaneous presence of both IL-4 and LPS for at least 24 h was required for a detectable IgE response. For an optimal IgE response, IL-4 needed to be present for more than 72 h in LPS-activated cultures. The possible reasons for the different regulation of IgG1 and IgE responses are discussed.
H B Moon, and
E Severinson, and
C Heusser, and
S G Johansson, and
G Möller, and
U Persson
January 1988,
The Journal of experimental medicine,
H B Moon, and
E Severinson, and
C Heusser, and
S G Johansson, and
G Möller, and
U Persson
February 1988,
Immunological reviews,
H B Moon, and
E Severinson, and
C Heusser, and
S G Johansson, and
G Möller, and
U Persson
November 1992,
European journal of immunology,
H B Moon, and
E Severinson, and
C Heusser, and
S G Johansson, and
G Möller, and
U Persson
July 1989,
European journal of immunology,
H B Moon, and
E Severinson, and
C Heusser, and
S G Johansson, and
G Möller, and
U Persson
May 1993,
Immunology,
H B Moon, and
E Severinson, and
C Heusser, and
S G Johansson, and
G Möller, and
U Persson
April 1993,
Proceedings of the National Academy of Sciences of the United States of America,
H B Moon, and
E Severinson, and
C Heusser, and
S G Johansson, and
G Möller, and
U Persson
September 1994,
Allergy,
H B Moon, and
E Severinson, and
C Heusser, and
S G Johansson, and
G Möller, and
U Persson
September 1994,
Cellular immunology,
H B Moon, and
E Severinson, and
C Heusser, and
S G Johansson, and
G Möller, and
U Persson
January 1989,
International archives of allergy and applied immunology,
H B Moon, and
E Severinson, and
C Heusser, and
S G Johansson, and
G Möller, and
U Persson
August 1984,
Journal of immunology (Baltimore, Md. : 1950),
