Production of recombinant human epidermal growth factor in Pichia pastoris. 2017

Samira Eissazadeh, and Hassan Moeini, and Maryam Ghandizadeh Dezfouli, and Somayyeh Heidary, and Rubina Nelofer, and Mohd Puad Abdullah
Universiti Putra Malaysia, Faculty of Biotechnology and Molecular Science, Department of Cellular and Molecular Biology, Malaysia.

This study was carried out to express human epidermal growth factor (hEGF) in Pichia pastoris GS115. For this aim, the hEGF gene was cloned into the pPIC9K expression vector, and then integrated into P. pastoris by electroporation. ELISA-based assay showed that the amount of hEGF secreted into the medium can be affected by the fermentation conditions especially by culture medium, pH and temperature. The best medium for the optimal hEGF production was BMMY buffered at a pH range of 6.0 and 7.0. The highest amount of hEGF with an average yield of 2.27μg/mL was obtained through an induction of the culture with 0.5% (v/v) methanol for 60h. The artificial neural network (ANN) analysis revealed that changes in both pH and temperature significantly affected the hEGF production with the pH change had slightly higher impact on hEGF production than variations in the temperature.

UI MeSH Term Description Entries
D010843 Pichia Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap. Hansenula,Hansenulas,Pichias
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D004815 Epidermal Growth Factor A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form. EGF,Epidermal Growth Factor-Urogastrone,Urogastrone,Human Urinary Gastric Inhibitor,beta-Urogastrone,Growth Factor, Epidermal,Growth Factor-Urogastrone, Epidermal,beta Urogastrone
D005285 Fermentation Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID. Fermentations
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D015870 Gene Expression The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. Expression, Gene,Expressions, Gene,Gene Expressions

Related Publications

Samira Eissazadeh, and Hassan Moeini, and Maryam Ghandizadeh Dezfouli, and Somayyeh Heidary, and Rubina Nelofer, and Mohd Puad Abdullah
November 2005, Biotechnology letters,
Samira Eissazadeh, and Hassan Moeini, and Maryam Ghandizadeh Dezfouli, and Somayyeh Heidary, and Rubina Nelofer, and Mohd Puad Abdullah
November 2021, Sheng wu gong cheng xue bao = Chinese journal of biotechnology,
Samira Eissazadeh, and Hassan Moeini, and Maryam Ghandizadeh Dezfouli, and Somayyeh Heidary, and Rubina Nelofer, and Mohd Puad Abdullah
March 2005, Journal of bioscience and bioengineering,
Samira Eissazadeh, and Hassan Moeini, and Maryam Ghandizadeh Dezfouli, and Somayyeh Heidary, and Rubina Nelofer, and Mohd Puad Abdullah
January 2000, Journal of bioscience and bioengineering,
Samira Eissazadeh, and Hassan Moeini, and Maryam Ghandizadeh Dezfouli, and Somayyeh Heidary, and Rubina Nelofer, and Mohd Puad Abdullah
October 2003, Bioscience, biotechnology, and biochemistry,
Samira Eissazadeh, and Hassan Moeini, and Maryam Ghandizadeh Dezfouli, and Somayyeh Heidary, and Rubina Nelofer, and Mohd Puad Abdullah
May 2002, Sheng wu gong cheng xue bao = Chinese journal of biotechnology,
Samira Eissazadeh, and Hassan Moeini, and Maryam Ghandizadeh Dezfouli, and Somayyeh Heidary, and Rubina Nelofer, and Mohd Puad Abdullah
October 2007, Journal of biotechnology,
Samira Eissazadeh, and Hassan Moeini, and Maryam Ghandizadeh Dezfouli, and Somayyeh Heidary, and Rubina Nelofer, and Mohd Puad Abdullah
March 2014, Molecular biology reports,
Samira Eissazadeh, and Hassan Moeini, and Maryam Ghandizadeh Dezfouli, and Somayyeh Heidary, and Rubina Nelofer, and Mohd Puad Abdullah
July 2019, Bulletin of experimental biology and medicine,
Samira Eissazadeh, and Hassan Moeini, and Maryam Ghandizadeh Dezfouli, and Somayyeh Heidary, and Rubina Nelofer, and Mohd Puad Abdullah
November 2001, Protein expression and purification,
Copied contents to your clipboard!