BIOMAT Database Logo BIOMAT Database Logo

The fatty acid-binding protein from human skeletal muscle. 1989

R A Peeters, and M A in't Groen, and J H Veerkamp
Department of Biochemistry, University of Nijmegen, The Netherlands.

Fatty acid-binding protein (FABP) was isolated from human skeletal muscle by gel filtration and anion- and cation-exchange chromatography. The isolation procedure, however, with rat and pig skeletal muscle gave mostly inactive preparations. Rat muscle FABP preparations contained parvalbumin as a contaminant. FABP from human muscle had a Mr of about 15 kDa, a pI value of 5.2, and a Kd value with oleic acid of 0.50 microM. Skeletal muscle and heart FABPs and their antisera showed a strong cross-reactivity on Western blots and in enzyme-linked immunosorbent assays (ELISA). No cross-reactivity was observed with liver FABP and its antiserum. On the basis of amino acid composition, electrophoretic behavior, fatty acid binding, and immunochemical properties, human skeletal muscle FABP must be similar or closely related to human heart FABP. The FABP content determined by ELISA was comparable in various human muscles and cultured muscle cells, but lower than that in rat muscles.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D009124 Muscle Proteins The protein constituents of muscle, the major ones being ACTINS and MYOSINS. More than a dozen accessory proteins exist including TROPONIN; TROPOMYOSIN; and DYSTROPHIN. Muscle Protein,Protein, Muscle,Proteins, Muscle
D009132 Muscles Contractile tissue that produces movement in animals. Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D009363 Neoplasm Proteins Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm. Proteins, Neoplasm
D009419 Nerve Tissue Proteins Proteins, Nerve Tissue,Tissue Proteins, Nerve
D002352 Carrier Proteins Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes. Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D005227 Fatty Acids Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed) Aliphatic Acid,Esterified Fatty Acid,Fatty Acid,Fatty Acids, Esterified,Fatty Acids, Saturated,Saturated Fatty Acid,Aliphatic Acids,Acid, Aliphatic,Acid, Esterified Fatty,Acid, Saturated Fatty,Esterified Fatty Acids,Fatty Acid, Esterified,Fatty Acid, Saturated,Saturated Fatty Acids
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

R A Peeters, and M A in't Groen, and J H Veerkamp
Fatty acid binding protein 4 in human skeletal muscle.
July 2006, Biochemical and biophysical research communications,
R A Peeters, and M A in't Groen, and J H Veerkamp
Cytoplasmic fatty acid-binding protein facilitates fatty acid utilization by skeletal muscle.
August 2003, Acta physiologica Scandinavica,
R A Peeters, and M A in't Groen, and J H Veerkamp
The membrane-associated 40 KD fatty acid binding protein (Berk's protein), a putative fatty acid transporter is present in human skeletal muscle.
January 1996, Life sciences,
R A Peeters, and M A in't Groen, and J H Veerkamp
Muscle fatty acid-binding protein.
November 1999, Biochimica et biophysica acta,
R A Peeters, and M A in't Groen, and J H Veerkamp
Fatty acid binding protein facilitates sarcolemmal fatty acid transport but not mitochondrial oxidation in rat and human skeletal muscle.
July 2007, The Journal of physiology,
R A Peeters, and M A in't Groen, and J H Veerkamp
Studies of plasma membrane fatty acid-binding protein and other lipid-binding proteins in human skeletal muscle.
May 2004, The Proceedings of the Nutrition Society,
R A Peeters, and M A in't Groen, and J H Veerkamp
Fatty acid binding and conformational stability of mutants of human muscle fatty acid-binding protein.
February 1996, The Biochemical journal,
R A Peeters, and M A in't Groen, and J H Veerkamp
Membrane associated fatty acid binding protein (FABPpm) in human skeletal muscle is increased by endurance training.
February 1997, Biochemical and biophysical research communications,
R A Peeters, and M A in't Groen, and J H Veerkamp
Fatty acid oxidation in human skeletal muscle.
December 2002, The Journal of clinical investigation,
R A Peeters, and M A in't Groen, and J H Veerkamp
Fatty-acid-binding protein from the flight muscle of Locusta migratoria: evolutionary variations in fatty acid binding.
May 2006, Biochemistry,
Copied contents to your clipboard!