Laser reflectance measurement for the online monitoring of Chlorella sorokiniana biomass concentration. 2017

Patricio López Expósito, and Angeles Blanco Suárez, and Carlos Negro Álvarez
Chemical Engineering Department, Complutentse University of Madrid, Faculty of Chemical Sciences, Avda. Complutense s/n, Madrid 28040, Spain. Electronic address: plopezex@ucm.es.

Fast and reliable methods to determine biomass concentration are necessary to facilitate the large scale production of microalgae. A method for the rapid estimation of Chlorella sorokiniana biomass concentration was developed. The method translates the suspension particle size spectrum gathered though laser reflectance into biomass concentration by means of two machine learning modelling techniques. In each case, the model hyper-parameters were selected applying a simulated annealing algorithm. The results show that dry biomass concentration can be estimated with a very good accuracy (R2=0.87). The presented method seems to be suited to perform fast estimations of biomass concentration in suspensions of microalgae cultivated in moderately turbid media with tendency to aggregate.

UI MeSH Term Description Entries
D007834 Lasers An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum. Masers,Continuous Wave Lasers,Pulsed Lasers,Q-Switched Lasers,Continuous Wave Laser,Laser,Laser, Continuous Wave,Laser, Pulsed,Laser, Q-Switched,Lasers, Continuous Wave,Lasers, Pulsed,Lasers, Q-Switched,Maser,Pulsed Laser,Q Switched Lasers,Q-Switched Laser
D009862 Online Systems Computer-based Information systems having real-time remote access to information or processes. On-Line Systems,On Line Systems,On-Line System,Online System,System, On-Line,System, Online,Systems, On-Line,Systems, Online
D002138 Calibration Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output. Calibrations
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002708 Chlorella Nonmotile unicellular green algae potentially valuable as a source of high-grade protein and B-complex vitamins. Chlorellas
D004784 Environmental Monitoring The monitoring of the level of toxins, chemical pollutants, microbial contaminants, or other harmful substances in the environment (soil, air, and water), workplace, or in the bodies of people and animals present in that environment. Monitoring, Environmental,Environmental Surveillance,Surveillance, Environmental
D005427 Flocculation The aggregation of suspended solids into larger clumps. Flocculations
D001709 Biotechnology Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction. Biotechnologies
D058433 Photobioreactors Devices for generating biological products that use light as the energy source. They are used for controlled BIOMASS production such as growing cyanobacteria, mosses, or algae. Photobioreactor
D062065 Wastewater Contaminated water generated as a waste product of human activity. Waste Water,Waste Waters,Wastewaters,Water, Waste,Waters, Waste

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