BIOMAT Database Logo BIOMAT Database Logo

Effect of different semen extenders for the storage of chilled sperm in Tigrina (Leopardus tigrinus). 2017

D S R Angrimani, and P M H Barros, and J D A Losano, and C N M Cortada, and R P Bertolla, and M A B V Guimarães, and S H R Correa, and V H Barnabe, and M Nichi
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.

To enhance the conservation of endangered populations, the present study aimed to evaluate whether Tigrinas (Leopardus tigrinus) sperm could be conserved under refrigeration for short periods while maintaining sufficient quality for use in assisted-reproductive techniques (i.e., cryopreservation, in vitro fertilization). For this purpose, semen samples from 15 Tigrinas individuals were submitted to conventional and functional tests after different cooling periods (4 °C; 0, 12, and 24 hours postcooling), using TCM 199 (TCM), Ham's F10 (HAM), Ham's F10 with bovine serum albumin (HBSA), and Tris-citrate egg yolk (TEYC) extenders. In a second step, semen cooled using TEYC was supplemented with reduced glutathione (GSH) at different concentrations (0, 0.5, 1.0, and 1.5 mM). TEYC yielded superior results compared with TCM, HAM, and HBSA even after 24 hours of cooling in regard to the sperm motility index (SMI-TEYC: 50.2 ± 1.7%), high mitochondrial activity (TEYC: 51.4 ± 1.9%), plasma membrane integrity (TEYC: 53 ± 2.1%), and DNA integrity (TEYC: 56.3 ± 2.9%). In regard to the concentration of thiobarbituric-acid-reactive substances (TBARS), TEYC (1900.1 ± 341.4 ng/106 spermatozoa) showed higher levels compared with the other extenders (HAM: 638.7 ± 121.6 ng/106 spermatozoa; HBSA: 468.7 ± 95.6 ng/106 spermatozoa; TCM: 169.6 ± 31.6 ng/106 spermatozoa). However, GSH therapy had no effect. In conclusion, the TEYC extender may be useful in maintaining sperm parameters of Tigrinas for up to 24 hours at 4 °C. Furthermore, these results allow the transport of this material at a minimum quality to be further used for artificial insemination, in vitro fertilization, and the development of semen cryopreservation protocols.

UI MeSH Term Description Entries
D008297 Male Males
D012034 Refrigeration The mechanical process of cooling. Cold Chain,Chain, Cold,Chains, Cold,Cold Chains
D003451 Cryoprotective Agents Substances that provide protection against the harmful effects of freezing temperatures. Cryoprotective Agent,Cryoprotective Effect,Cryoprotective Effects,Agent, Cryoprotective,Agents, Cryoprotective,Effect, Cryoprotective,Effects, Cryoprotective
D004530 Egg Yolk Cytoplasm stored in an egg that contains nutritional reserves for the developing embryo. It is rich in polysaccharides, lipids, and proteins. Egg Yolks,Yolk, Egg,Yolks, Egg
D005978 Glutathione A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides. Reduced Glutathione,gamma-L-Glu-L-Cys-Gly,gamma-L-Glutamyl-L-Cysteinylglycine,Glutathione, Reduced,gamma L Glu L Cys Gly,gamma L Glutamyl L Cysteinylglycine
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012662 Semen Preservation The process by which semen is kept viable outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism). Frozen Semen,Sperm Preservation,Preservation, Semen,Preservation, Sperm,Semen, Frozen
D013081 Sperm Motility Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression. Motilities, Sperm,Motility, Sperm,Sperm Motilities
D013094 Spermatozoa Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility. Sperm,Spermatozoon,X-Bearing Sperm,X-Chromosome-Bearing Sperm,Y-Bearing Sperm,Y-Chromosome-Bearing Sperm,Sperm, X-Bearing,Sperm, X-Chromosome-Bearing,Sperm, Y-Bearing,Sperm, Y-Chromosome-Bearing,Sperms, X-Bearing,Sperms, X-Chromosome-Bearing,Sperms, Y-Bearing,Sperms, Y-Chromosome-Bearing,X Bearing Sperm,X Chromosome Bearing Sperm,X-Bearing Sperms,X-Chromosome-Bearing Sperms,Y Bearing Sperm,Y Chromosome Bearing Sperm,Y-Bearing Sperms,Y-Chromosome-Bearing Sperms
D045989 Felidae The cat family in the order CARNIVORA comprised of muscular, deep-chested terrestrial carnivores with a highly predatory lifestyle. Leopardus pardalis,Neofelis nebulosa,Ocelots,Leopards, Clouded,Clouded Leopard,Clouded Leopards,Leopard, Clouded,Ocelot

Related Publications

D S R Angrimani, and P M H Barros, and J D A Losano, and C N M Cortada, and R P Bertolla, and M A B V Guimarães, and S H R Correa, and V H Barnabe, and M Nichi
Longevity of chilled canine semen comparing different extenders.
November 2012, Animal reproduction science,
D S R Angrimani, and P M H Barros, and J D A Losano, and C N M Cortada, and R P Bertolla, and M A B V Guimarães, and S H R Correa, and V H Barnabe, and M Nichi
Effect of different extenders and storage temperatures on sperm viability of liquid ram semen.
January 2002, Theriogenology,
D S R Angrimani, and P M H Barros, and J D A Losano, and C N M Cortada, and R P Bertolla, and M A B V Guimarães, and S H R Correa, and V H Barnabe, and M Nichi
Effects of different extenders, storage temperatures, and antioxidant supplementation on chilled semen quality: a review.
February 2024, Tropical animal health and production,
D S R Angrimani, and P M H Barros, and J D A Losano, and C N M Cortada, and R P Bertolla, and M A B V Guimarães, and S H R Correa, and V H Barnabe, and M Nichi
Effect of housing and environmental enrichment on adrenocortical activity, behavior and reproductive cyclicity in the female tigrina (Leopardus tigrinus) and margay (Leopardus wiedii).
November 2007, Zoo biology,
D S R Angrimani, and P M H Barros, and J D A Losano, and C N M Cortada, and R P Bertolla, and M A B V Guimarães, and S H R Correa, and V H Barnabe, and M Nichi
Seasonal analysis of semen characteristics, serum testosterone and fecal androgens in the ocelot (Leopardus pardalis), margay (L. wiedii) and tigrina (L. tigrinus).
May 2002, Theriogenology,
D S R Angrimani, and P M H Barros, and J D A Losano, and C N M Cortada, and R P Bertolla, and M A B V Guimarães, and S H R Correa, and V H Barnabe, and M Nichi
The Effect of Sperm Concentration and Storage Vessel on Quercetin-Supplemented Rabbit Semen During Chilled Storage.
August 2015, Reproduction in domestic animals = Zuchthygiene,
D S R Angrimani, and P M H Barros, and J D A Losano, and C N M Cortada, and R P Bertolla, and M A B V Guimarães, and S H R Correa, and V H Barnabe, and M Nichi
Liquid storage of dromedary camel semen in different extenders.
August 2019, Animal reproduction science,
D S R Angrimani, and P M H Barros, and J D A Losano, and C N M Cortada, and R P Bertolla, and M A B V Guimarães, and S H R Correa, and V H Barnabe, and M Nichi
Liquid Storage of Geophagus brasiliensis semen in the Presence of Different Extenders.
January 2020, Cryo letters,
D S R Angrimani, and P M H Barros, and J D A Losano, and C N M Cortada, and R P Bertolla, and M A B V Guimarães, and S H R Correa, and V H Barnabe, and M Nichi
Genetic diversity of microsatellite loci in Leopardus pardalis, Leopardus wiedii and Leopardus tigrinus.
June 2007, Genetics and molecular research : GMR,
D S R Angrimani, and P M H Barros, and J D A Losano, and C N M Cortada, and R P Bertolla, and M A B V Guimarães, and S H R Correa, and V H Barnabe, and M Nichi
The effect of semen extenders and sperm number on mare fertility.
October 1975, Journal of reproduction and fertility. Supplement,
Copied contents to your clipboard!