Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Efficient Fabrication and Sealing of Chips Using a "Chip-Olate" Process. 2017

Yuksel Temiz, and Emmanuel Delamarche
IBM Research GmbH, Saumerstrasse 4, 8803, Rüschlikon, Switzerland.

The fabrication of silicon-based microfluidic chips is invaluable in supporting the development of many microfluidic concepts for research in the life sciences and in vitro diagnostic applications such as the realization of miniaturized immunoassays using capillary-driven chips. While being extremely abundant, the literature covering microfluidic chip fabrication and assay development might not have addressed properly the challenge of fabricating microfluidic chips on a wafer level or the need for dicing wafers to release chips that need then to be further processed, cleaned, rinsed, and dried one by one. Here, we describe the "chip-olate" process wherein microfluidic structures are formed on a silicon wafer, followed by partial dicing, cleaning, and drying steps. Then, integration of reagents (if any) can be done, followed by lamination of a sealing cover. Breaking by hand the partially diced wafer yields individual chips ready for use.

UI MeSH Term Description Entries
D007118 Immunoassay A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance. Immunochromatographic Assay,Assay, Immunochromatographic,Assays, Immunochromatographic,Immunoassays,Immunochromatographic Assays
D004867 Equipment Design Methods and patterns of fabricating machines and related hardware. Design, Equipment,Device Design,Medical Device Design,Design, Medical Device,Designs, Medical Device,Device Design, Medical,Device Designs, Medical,Medical Device Designs,Design, Device,Designs, Device,Designs, Equipment,Device Designs,Equipment Designs
D044085 Microfluidics The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES. Microfluidic
D046210 Microfluidic Analytical Techniques Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids. Microfluidic Analysis,Analyses, Microfluidic,Analysis, Microfluidic,Analytical Technique, Microfluidic,Analytical Techniques, Microfluidic,Microfluidic Analyses,Microfluidic Analytical Technique,Technique, Microfluidic Analytical,Techniques, Microfluidic Analytical

Related Publications

Yuksel Temiz, and Emmanuel Delamarche
September 2011, Biosensors & bioelectronics,
Yuksel Temiz, and Emmanuel Delamarche
January 2013, Annual review of analytical chemistry (Palo Alto, Calif.),
Yuksel Temiz, and Emmanuel Delamarche
July 2018, The Analyst,
Yuksel Temiz, and Emmanuel Delamarche
January 2011, Methods in molecular biology (Clifton, N.J.),
Yuksel Temiz, and Emmanuel Delamarche
January 2022, Biosensors & bioelectronics,
Yuksel Temiz, and Emmanuel Delamarche
December 2023, Small (Weinheim an der Bergstrasse, Germany),
Yuksel Temiz, and Emmanuel Delamarche
December 2020, Small (Weinheim an der Bergstrasse, Germany),
Yuksel Temiz, and Emmanuel Delamarche
November 2013, Accounts of chemical research,
Copied contents to your clipboard!