Biomaterial Database

Analysis of 18O enrichment in biological fluids by continuous flow-isotope ratio mass spectrometry. 1989

D C McMillan, and T Preston, and D P Taggart

University Department of Surgery, Royal Infirmary, Glasgow, UK.

A novel method is described for the analysis of 18O in urine by continuous flow-isotope ratio mass spectrometry (CF-IRMS), after sample equilibration with CO2. The method is shown to be fast, precise and accurate and therefore facilitates studies of total body water and water turnover in the clinical field. The method uses existing CF-IRMS instrumentation with minor hardware modification which does not compromise routine analysis of 13C and 15N. This method emphasizes the versatility of CF-IRMS and thus its economy for the biomedical research group using stable isotope tracers.

UI	MeSH Term	Description	Entries
D007553	Isotope Labeling	Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.	Isotope Labeling, Stable,Isotope-Coded Affinity Tagging,Isotopically-Coded Affinity Tagging,Affinity Tagging, Isotope-Coded,Affinity Tagging, Isotopically-Coded,Isotope Coded Affinity Tagging,Labeling, Isotope,Labeling, Stable Isotope,Stable Isotope Labeling,Tagging, Isotope-Coded Affinity,Tagging, Isotopically-Coded Affinity
D010100	Oxygen	An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.	Dioxygen,Oxygen-16,Oxygen 16
D010104	Oxygen Radioisotopes	Unstable isotopes of oxygen that decay or disintegrate emitting radiation. O atoms with atomic weights 13, 14, 15, 19, and 20 are radioactive oxygen isotopes.	Radioisotopes, Oxygen
D011312	Pressure	A type of stress exerted uniformly in all directions. Its measure is the force exerted per unit area. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Pressures
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013057	Spectrum Analysis	The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Spectroscopy,Analysis, Spectrum,Spectrometry