Biomaterial Database

Quantitative histofluorescence of hippocampal mossy fiber zinc. 1989

D D Savage, and C Y Montano, and E J Kasarskis

Department of Pharamacology, University of New Mexico School of Medicine, Albuquerque 87131.

N-(6-methoxy-8-quinolyl)-p-toluenesulfonamide (TS-Q) is a fluorescent probe for histochemically detecting a chelatable pool of zinc in the hippocampal formation and other brain regions. Utilization of zinc:TS-Q histofluorescence as a quantitative tool required further characterization of zinc:TS-Q fluorescence and the development of appropriate zinc standards. Studies of zinc:TS-Q fluorescence in aqueous solutions were conducted to optimize the reaction conditions for TS-Q chelation with zinc. Possible interference of zinc:TS-Q fluorescence by chelation of other biologically significant cations with zinc:TS-Q was shown to be negligible. Zinc standards were developed using a water soluble plastic embedding medium. Varying the concentration of zinc in the plastic sections yielded a standard curve exhibiting a log-linear relationship between 0.15 and 11 fg zinc/100 microns 2. Zinc:TS-Q histofluorescence was characterized in horizontal sections of rat ventral hippocampal formation using a computerized image analysis system. Section thickness and TS-Q concentration were optimized. The concentration of zinc varied between 1.5 and 17 fg/100 microns 2 in different regions of the mossy fiber projection of the ventral hippocampal formation. The development of this quantitative histofluorescence technique should facilitate our understanding of the functional role of zinc in the mossy fiber projection and can be utilized to investigate changes in mossy fiber zinc in a variety of pathological states.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D011919	Rats, Inbred Strains	Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.	August Rats,Inbred Rat Strains,Inbred Strain of Rat,Inbred Strain of Rats,Inbred Strains of Rats,Rat, Inbred Strain,August Rat,Inbred Rat Strain,Inbred Strain Rat,Inbred Strain Rats,Inbred Strains Rat,Inbred Strains Rats,Rat Inbred Strain,Rat Inbred Strains,Rat Strain, Inbred,Rat Strains, Inbred,Rat, August,Rat, Inbred Strains,Rats Inbred Strain,Rats Inbred Strains,Rats, August,Rats, Inbred Strain,Strain Rat, Inbred,Strain Rats, Inbred,Strain, Inbred Rat,Strains, Inbred Rat
D005456	Fluorescent Dyes	Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.	Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D006624	Hippocampus	A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.	Ammon Horn,Cornu Ammonis,Hippocampal Formation,Subiculum,Ammon's Horn,Hippocampus Proper,Ammons Horn,Formation, Hippocampal,Formations, Hippocampal,Hippocampal Formations,Hippocampus Propers,Horn, Ammon,Horn, Ammon's,Proper, Hippocampus,Propers, Hippocampus,Subiculums
D006651	Histocytochemistry	Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.	Cytochemistry
D000634	Aminoquinolines	Quinolines substituted in any position by one or more amino groups.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013449	Sulfonamides	A group of compounds that contain the structure SO2NH2.	Sulfonamide,Sulfonamide Mixture,Sulfonamide Mixtures,Mixture, Sulfonamide,Mixtures, Sulfonamide
D014105	Tosyl Compounds	Toluenesulfonyl compounds with general formula CH3C6H4S(O2)R” widely used to block amino groups in the course of syntheses of drugs and other biologically active compounds.	Toluenesulfonyl Compounds,Compounds, Toluenesulfonyl,Compounds, Tosyl
D015032	Zinc	A metallic element of atomic number 30 and atomic weight 65.38. It is a necessary trace element in the diet, forming an essential part of many enzymes, and playing an important role in protein synthesis and in cell division. Zinc deficiency is associated with ANEMIA, short stature, HYPOGONADISM, impaired WOUND HEALING, and geophagia. It is known by the symbol Zn.