Calcium Detection in Secretion Granules of Avian Oviduct by Scanning Electron Microscopy (SEM) and Energy-dispersive X-Ray Microanalysis (EDX) 1. 1983

Takashi Makita, and Masanori Hatsuoka, and Yukiko Sugi
Department of Veterinary Anatomy (T.M; M.H.), Yamaguchi University, Yamaguchi City, and Department of Anatomy (Y.S.), Saitama Medical College, Japan (OA 82-281P1).

Secretion granules in the shell gland, isthmus, and albumin-secreting region of the hen oviduct were analyzed with WET-scanning electron microscopy (SEM) and EDX, a combination of wide-angle backscattered electron detector (BED) and energy-dispersive X-ray microanalyzer (EDX). Glutaraldehyde-fixed but unhydrated, unstained, and uncoated samples were analyzed; Ca was localized in all secretion granules in all three sections of the hen oviduct studied.

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