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Improving the Resolution of the 2-Deoxy-D-Glucose Method 1 , 2. 1981

Ch Pilgrim, and H-J Wagner
Abteilung für Klinische Morphologie der Universität Ulm, Oberer Eselsberg, D-7900 Ulm, Federal Republic of Germany.

A modified thaw-mount technique is described for the autoradiographic localization of 2-(3H) deoxy-D-glucose in the rat brain. The demonstration of highly labeled individual cell bodies in characteristic sites proves that a resolution on the cellular level has been achieved. Attempts to recover these cells in autoradiographs of semithin sections after resin embedding by vascular perfusion of fixatives, dehydrating, and embedding media were unsuccessful.

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