IL-33 acts as a foe to MIA PaCa-2 pancreatic cancer. 2017

Yujiang Fang, and Lei Zhao, and Huaping Xiao, and Kathryn M Cook, and Qian Bai, and Elizabeth J Herrick, and Xuhui Chen, and Chenglu Qin, and Ziwen Zhu, and Mark R Wakefield, and Michael B Nicholl
Department of Microbiology, Immunology and Pathology, College of Osteopathic Medicine, Des Moines University, Des Moines, IA, 50312, USA. yujiang.fang@dmu.edu.

IL-33 is a member of the IL-1 family of cytokines, and no study has been performed to address its direct anti-tumor effect. This study is designed to investigate whether IL-33 has any direct effect on pancreatic cancer. Clonogenic survival assay, immunohistochemistry, TUNEL staining, proliferation, caspase-3 activity kits and RT-PCR were used to evaluate the effects of IL-33 on cell survival, proliferation and apoptosis of a pancreatic cancer cell line, MIA PaCa-2. We found that the percentage of colonies of MIA PaCa-2 cells, PCNA+ cells and the OD value of cancer cells were all decreased in the presence of IL-33. TUNEL+ cells and the relative caspase-3 activity in cancer cells were increased in the presence of IL-33. We further found that its anti-proliferative effect on cancer cells correlated with downregulation of pro-proliferative molecules cdk2 and cdk4 and upregulation of anti-proliferative molecules p15, p21 and p53. Its pro-apoptotic effect correlated with downregulation of anti-apoptotic molecule FLIP and upregulation of pro-apoptotic molecule TRAIL. These results suggest that IL-33 presents significant anti-tumor effects by inhibition of proliferation and induction of apoptosis of MIA PaCa-2 pancreatic cancer cells. Thus, strength of IL-33/ST2 signal pathway might be a promising way to treat pancreatic cancer.

UI MeSH Term Description Entries
D010190 Pancreatic Neoplasms Tumors or cancer of the PANCREAS. Depending on the types of ISLET CELLS present in the tumors, various hormones can be secreted: GLUCAGON from PANCREATIC ALPHA CELLS; INSULIN from PANCREATIC BETA CELLS; and SOMATOSTATIN from the SOMATOSTATIN-SECRETING CELLS. Most are malignant except the insulin-producing tumors (INSULINOMA). Cancer of Pancreas,Pancreatic Cancer,Cancer of the Pancreas,Neoplasms, Pancreatic,Pancreas Cancer,Pancreas Neoplasms,Pancreatic Acinar Carcinoma,Pancreatic Carcinoma,Acinar Carcinoma, Pancreatic,Acinar Carcinomas, Pancreatic,Cancer, Pancreas,Cancer, Pancreatic,Cancers, Pancreas,Cancers, Pancreatic,Carcinoma, Pancreatic,Carcinoma, Pancreatic Acinar,Carcinomas, Pancreatic,Carcinomas, Pancreatic Acinar,Neoplasm, Pancreas,Neoplasm, Pancreatic,Neoplasms, Pancreas,Pancreas Cancers,Pancreas Neoplasm,Pancreatic Acinar Carcinomas,Pancreatic Cancers,Pancreatic Carcinomas,Pancreatic Neoplasm
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000067596 Interleukin-33 A member of the INTERLEUKIN-1 protein family involved in the maturation of TH2 CELLS and the activation of MAST CELLS; BASOPHILS; EOSINOPHILS and NK CELLS. It is also produced by ENDOTHELIAL CELLS; EPITHELIAL CELLS and FIBROBLASTS; where it can function as an alarmin to modulate immune and inflammatory responses to tissue damage. IL-33,IL33,Interleukin 33
D000072179 Interleukin-1 Receptor-Like 1 Protein A receptor for INTERLEUKIN-33 that is related structurally to the interleukin-1 receptor. It contains three extracellular IMMUNOGLOBULIN-LIKE DOMAIN regions and associates with INTERLEUKIN-1 RECEPTOR ACCESSORY PROTEIN upon binding IL-33 to initiate signaling. It may function in the response of HELPER T CELLS to INFLAMMATION. IL1RL1 Protein,Interleukin 1 Receptor-Related Protein,Interleukin-33 Receptor,Interleukin 1 Receptor Like 1 Protein,Interleukin 1 Receptor Related Protein,Interleukin 33 Receptor,Receptor, Interleukin-33
D017209 Apoptosis A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines
D048708 Cell Growth Processes Processes required for CELL ENLARGEMENT and CELL PROLIFERATION. Processes, Cell Growth

Related Publications

Yujiang Fang, and Lei Zhao, and Huaping Xiao, and Kathryn M Cook, and Qian Bai, and Elizabeth J Herrick, and Xuhui Chen, and Chenglu Qin, and Ziwen Zhu, and Mark R Wakefield, and Michael B Nicholl
January 1977, International journal of cancer,
Yujiang Fang, and Lei Zhao, and Huaping Xiao, and Kathryn M Cook, and Qian Bai, and Elizabeth J Herrick, and Xuhui Chen, and Chenglu Qin, and Ziwen Zhu, and Mark R Wakefield, and Michael B Nicholl
January 2012, Asian Pacific journal of cancer prevention : APJCP,
Yujiang Fang, and Lei Zhao, and Huaping Xiao, and Kathryn M Cook, and Qian Bai, and Elizabeth J Herrick, and Xuhui Chen, and Chenglu Qin, and Ziwen Zhu, and Mark R Wakefield, and Michael B Nicholl
July 2003, British journal of cancer,
Yujiang Fang, and Lei Zhao, and Huaping Xiao, and Kathryn M Cook, and Qian Bai, and Elizabeth J Herrick, and Xuhui Chen, and Chenglu Qin, and Ziwen Zhu, and Mark R Wakefield, and Michael B Nicholl
January 2015, International journal of clinical and experimental medicine,
Yujiang Fang, and Lei Zhao, and Huaping Xiao, and Kathryn M Cook, and Qian Bai, and Elizabeth J Herrick, and Xuhui Chen, and Chenglu Qin, and Ziwen Zhu, and Mark R Wakefield, and Michael B Nicholl
October 1993, Surgical oncology,
Yujiang Fang, and Lei Zhao, and Huaping Xiao, and Kathryn M Cook, and Qian Bai, and Elizabeth J Herrick, and Xuhui Chen, and Chenglu Qin, and Ziwen Zhu, and Mark R Wakefield, and Michael B Nicholl
June 1990, Gastroenterology,
Yujiang Fang, and Lei Zhao, and Huaping Xiao, and Kathryn M Cook, and Qian Bai, and Elizabeth J Herrick, and Xuhui Chen, and Chenglu Qin, and Ziwen Zhu, and Mark R Wakefield, and Michael B Nicholl
October 1995, Research communications in molecular pathology and pharmacology,
Yujiang Fang, and Lei Zhao, and Huaping Xiao, and Kathryn M Cook, and Qian Bai, and Elizabeth J Herrick, and Xuhui Chen, and Chenglu Qin, and Ziwen Zhu, and Mark R Wakefield, and Michael B Nicholl
January 1985, FEBS letters,
Yujiang Fang, and Lei Zhao, and Huaping Xiao, and Kathryn M Cook, and Qian Bai, and Elizabeth J Herrick, and Xuhui Chen, and Chenglu Qin, and Ziwen Zhu, and Mark R Wakefield, and Michael B Nicholl
November 2022, International journal of molecular sciences,
Yujiang Fang, and Lei Zhao, and Huaping Xiao, and Kathryn M Cook, and Qian Bai, and Elizabeth J Herrick, and Xuhui Chen, and Chenglu Qin, and Ziwen Zhu, and Mark R Wakefield, and Michael B Nicholl
March 1998, Pancreas,
Copied contents to your clipboard!