BIOMAT Database Logo BIOMAT Database Logo

Demonstration of two distinct insulin-binding components in solubilized human placental membranes by radioimmunoassay using synthetic peptide and anti-synthetic peptide antibody. 1989

N Yanaihara, and C Yanaihara, and K Iguchi, and T Inoue, and M Toyoshige, and T Mochizuki, and M Hoshino, and N Kurokawa, and T Kaneko
University of Shizuoka School of Pharmaceutical Sciences, Japan.

A peptide corresponding to the 957-980 sequence of human placental insulin receptor precursor (HIRP) was synthesized and antisera were produced against the synthetic peptide. Anti-synthetic HIRP(957-980) serum HIR-27 was proved to cross-react with HIRP-related proteins in solubilized human placental membranes. A radioimmunoassay developed with the antiserum and synthetic peptide HIRP(957-980) enabled us to separate, in combination with gel filtration, two insulin-binding components in solubilized human placental membranes which conceivably correspond to the alpha 2 beta 2 and alpha beta structures of the placental insulin receptor. The two components were shown to be distinct in insulin-binding behavior depending on conditions of pH and ionic strength in the binding assay.

UI MeSH Term Description Entries
D007106 Immune Sera Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen. Antisera,Immune Serums,Sera, Immune,Serums, Immune
D007700 Kinetics The rate dynamics in chemical or physical systems.
D010455 Peptides Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS. Peptide,Polypeptide,Polypeptides
D010920 Placenta A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES). Placentoma, Normal,Placentome,Placentas,Placentomes
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D011972 Receptor, Insulin A cell surface receptor for INSULIN. It comprises a tetramer of two alpha and two beta subunits which are derived from cleavage of a single precursor protein. The receptor contains an intrinsic TYROSINE KINASE domain that is located within the beta subunit. Activation of the receptor by INSULIN results in numerous metabolic changes including increased uptake of GLUCOSE into the liver, muscle, and ADIPOSE TISSUE. Insulin Receptor,Insulin Receptor Protein-Tyrosine Kinase,Insulin Receptor alpha Subunit,Insulin Receptor beta Subunit,Insulin Receptor alpha Chain,Insulin Receptor beta Chain,Insulin-Dependent Tyrosine Protein Kinase,Receptors, Insulin,Insulin Receptor Protein Tyrosine Kinase,Insulin Receptors
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

N Yanaihara, and C Yanaihara, and K Iguchi, and T Inoue, and M Toyoshige, and T Mochizuki, and M Hoshino, and N Kurokawa, and T Kaneko
Glucocorticoid binding to solubilized components of human placental trophoblast membranes.
June 1980, Life sciences,
N Yanaihara, and C Yanaihara, and K Iguchi, and T Inoue, and M Toyoshige, and T Mochizuki, and M Hoshino, and N Kurokawa, and T Kaneko
The binding and degradation of insulin by rat liver membranes: demonstration of three distinct insulin-binding components in detergent-solubilized material from liver plasma membrane.
January 1980, Endocrinology,
N Yanaihara, and C Yanaihara, and K Iguchi, and T Inoue, and M Toyoshige, and T Mochizuki, and M Hoshino, and N Kurokawa, and T Kaneko
Radioimmunoassay of human proinsulin C-peptide using synthetic human connecting peptide.
April 1974, Endocrinologia japonica,
N Yanaihara, and C Yanaihara, and K Iguchi, and T Inoue, and M Toyoshige, and T Mochizuki, and M Hoshino, and N Kurokawa, and T Kaneko
Insulin binding to solubilized material from fat cell membranes: evidence for two binding species.
June 1978, Proceedings of the National Academy of Sciences of the United States of America,
N Yanaihara, and C Yanaihara, and K Iguchi, and T Inoue, and M Toyoshige, and T Mochizuki, and M Hoshino, and N Kurokawa, and T Kaneko
A novel radioimmunoassay for human erythropoietin using a synthetic NH2-terminal polypeptide and anti-peptide antibodies.
April 1984, Journal of immunological methods,
N Yanaihara, and C Yanaihara, and K Iguchi, and T Inoue, and M Toyoshige, and T Mochizuki, and M Hoshino, and N Kurokawa, and T Kaneko
Characterization of two insulin-binding components of rat-liver plasma membranes.
October 1982, Bioscience reports,
N Yanaihara, and C Yanaihara, and K Iguchi, and T Inoue, and M Toyoshige, and T Mochizuki, and M Hoshino, and N Kurokawa, and T Kaneko
[Detection of anti-HBAg by solid phase radioimmunoassay using labeled human antibody].
June 1975, Quaderni Sclavo di diagnostica clinica e di laboratorio,
N Yanaihara, and C Yanaihara, and K Iguchi, and T Inoue, and M Toyoshige, and T Mochizuki, and M Hoshino, and N Kurokawa, and T Kaneko
A solid-state competitive binding radioimmunoassay for measurement of antigens solubilized from membranes.
January 1976, Journal of immunological methods,
N Yanaihara, and C Yanaihara, and K Iguchi, and T Inoue, and M Toyoshige, and T Mochizuki, and M Hoshino, and N Kurokawa, and T Kaneko
Detection of anti-HBs in two groups of institutionalized people by solid-phase radioimmunoassay using human labelled antibody.
January 1975, Developments in biological standardization,
N Yanaihara, and C Yanaihara, and K Iguchi, and T Inoue, and M Toyoshige, and T Mochizuki, and M Hoshino, and N Kurokawa, and T Kaneko
Description of a human placental anti-insulin receptor polyclonal antibody that activates insulin binding to the receptor in vitro.
September 1988, Oncogene,
Copied contents to your clipboard!