BIOMAT Database Logo BIOMAT Database Logo

Aspartate inhibits LPS-induced MAFbx and MuRF1 expression in skeletal muscle in weaned pigs by regulating Akt, AMPKα and FOXO1. 2017

Yulan Liu, and Xiuying Wang, and Weibo Leng, and Dingan Pi, and Zhixiao Tu, and Huiling Zhu, and Haifeng Shi, and Shuang Li, and Yongqing Hou, and Chien-An Andy Hu
1 Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, People's Republic of China.

Infection and inflammation can result in the rapid loss of muscle mass and myofibrillar proteins (muscle atrophy). In addition, aspartate (Asp) is necessary for protein synthesis in mammalian cells. We hypothesized that Asp could attenuate LPS-induced muscle atrophy in a piglet model. Twenty-four weaning piglets were allotted to four treatments, including non-challenged control, LPS challenged control, LPS+0.5% Asp and LPS+1.0% Asp. On d 21, the piglets were injected with i.p. injection of LPS (100 ug/kg BM) or saline. At 4 h post-injection, blood, gastrocnemius and longissimus dorsi muscles samples were collected for biochemical and molecular analyses. Asp decreased the concentrations of cortisol and glucagon in plasma. In addition, Asp increased protein and RNA contents in muscles, and decreased mRNA expression of muscle atrophy F-box (MAFbx) and muscle RING finger 1 (MuRF1). Moreover, Asp decreased phosphorylation of AMPKα but increased phosphorylation of Akt and Forkhead Box O (FOXO) 1 in the muscles. Our results indicate that Asp suppresses LPS-induced MAFbx and MuRF1 expression via activation of Akt signaling, and inhibition of AMPKα and FOXO1 signaling.

UI MeSH Term Description Entries
D008070 Lipopolysaccharides Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed) Lipopolysaccharide,Lipoglycans
D009133 Muscular Atrophy Derangement in size and number of muscle fibers occurring with aging, reduction in blood supply, or following immobilization, prolonged weightlessness, malnutrition, and particularly in denervation. Atrophy, Muscle,Neurogenic Muscular Atrophy,Neurotrophic Muscular Atrophy,Atrophies, Muscle,Atrophies, Muscular,Atrophies, Neurogenic Muscular,Atrophies, Neurotrophic Muscular,Atrophy, Muscular,Atrophy, Neurogenic Muscular,Atrophy, Neurotrophic Muscular,Muscle Atrophies,Muscle Atrophy,Muscular Atrophies,Muscular Atrophies, Neurogenic,Muscular Atrophies, Neurotrophic,Muscular Atrophy, Neurogenic,Muscular Atrophy, Neurotrophic,Neurogenic Muscular Atrophies,Neurotrophic Muscular Atrophies
D011494 Protein Kinases A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein. Protein Kinase,Kinase, Protein,Kinases, Protein
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004195 Disease Models, Animal Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases. Animal Disease Model,Animal Disease Models,Disease Model, Animal
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D005934 Glucagon A 29-amino acid pancreatic peptide derived from proglucagon which is also the precursor of intestinal GLUCAGON-LIKE PEPTIDES. Glucagon is secreted by PANCREATIC ALPHA CELLS and plays an important role in regulation of BLOOD GLUCOSE concentration, ketone metabolism, and several other biochemical and physiological processes. (From Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th ed, p1511) Glucagon (1-29),Glukagon,HG-Factor,Hyperglycemic-Glycogenolytic Factor,Proglucagon (33-61),HG Factor,Hyperglycemic Glycogenolytic Factor
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006854 Hydrocortisone The main glucocorticoid secreted by the ADRENAL CORTEX. Its synthetic counterpart is used, either as an injection or topically, in the treatment of inflammation, allergy, collagen diseases, asthma, adrenocortical deficiency, shock, and some neoplastic conditions. Cortef,Cortisol,Pregn-4-ene-3,20-dione, 11,17,21-trihydroxy-, (11beta)-,11-Epicortisol,Cortifair,Cortril,Epicortisol,Hydrocortisone, (11 alpha)-Isomer,Hydrocortisone, (9 beta,10 alpha,11 alpha)-Isomer,11 Epicortisol
D000070584 Tripartite Motif Proteins A protein family defined by the presence of three ZINC FINGER domains, one of which is a RING FINGER DOMAIN, a coiled-coil region, and a highly variable C-terminal region. They function in many cellular processes including APOPTOSIS and CELL CYCLE regulation. RBCC Protein,TRIM Protein,Tripartite Motif Protein,RBCC Protein Family,RBCC Proteins,TRIM Protein Family,TRIM Proteins,Family, RBCC Protein,Family, TRIM Protein,Motif Protein, Tripartite,Motif Proteins, Tripartite,Protein Family, RBCC,Protein Family, TRIM,Protein, RBCC,Protein, TRIM,Protein, Tripartite Motif,Proteins, RBCC,Proteins, TRIM,Proteins, Tripartite Motif

Related Publications

Yulan Liu, and Xiuying Wang, and Weibo Leng, and Dingan Pi, and Zhixiao Tu, and Huiling Zhu, and Haifeng Shi, and Shuang Li, and Yongqing Hou, and Chien-An Andy Hu
The mERG1a channel modulates skeletal muscle MuRF1, but not MAFbx, expression.
March 2014, Muscle & nerve,
Yulan Liu, and Xiuying Wang, and Weibo Leng, and Dingan Pi, and Zhixiao Tu, and Huiling Zhu, and Haifeng Shi, and Shuang Li, and Yongqing Hou, and Chien-An Andy Hu
Imoxin attenuates LPS-induced inflammation and MuRF1 expression in mouse skeletal muscle.
December 2018, Physiological reports,
Yulan Liu, and Xiuying Wang, and Weibo Leng, and Dingan Pi, and Zhixiao Tu, and Huiling Zhu, and Haifeng Shi, and Shuang Li, and Yongqing Hou, and Chien-An Andy Hu
Ubiquitin ligases MuRF1 and MAFbx in human skeletal muscle atrophy.
January 2008, Joint bone spine,
Yulan Liu, and Xiuying Wang, and Weibo Leng, and Dingan Pi, and Zhixiao Tu, and Huiling Zhu, and Haifeng Shi, and Shuang Li, and Yongqing Hou, and Chien-An Andy Hu
Effects of fasting and refeeding on expression of MAFbx and MuRF1 in chick skeletal muscle.
October 2011, Science China. Life sciences,
Yulan Liu, and Xiuying Wang, and Weibo Leng, and Dingan Pi, and Zhixiao Tu, and Huiling Zhu, and Haifeng Shi, and Shuang Li, and Yongqing Hou, and Chien-An Andy Hu
αMSH blunts endotoxin-induced MuRF1 and atrogin-1 upregulation in skeletal muscle by modulating NF-κB and Akt/FoxO1 pathway.
January 2014, Mediators of inflammation,
Yulan Liu, and Xiuying Wang, and Weibo Leng, and Dingan Pi, and Zhixiao Tu, and Huiling Zhu, and Haifeng Shi, and Shuang Li, and Yongqing Hou, and Chien-An Andy Hu
Skeletal muscle atrophy and the E3 ubiquitin ligases MuRF1 and MAFbx/atrogin-1.
September 2014, American journal of physiology. Endocrinology and metabolism,
Yulan Liu, and Xiuying Wang, and Weibo Leng, and Dingan Pi, and Zhixiao Tu, and Huiling Zhu, and Haifeng Shi, and Shuang Li, and Yongqing Hou, and Chien-An Andy Hu
Ghrelin inhibits skeletal muscle protein breakdown in rats with thermal injury through normalizing elevated expression of E3 ubiquitin ligases MuRF1 and MAFbx.
April 2009, American journal of physiology. Regulatory, integrative and comparative physiology,
Yulan Liu, and Xiuying Wang, and Weibo Leng, and Dingan Pi, and Zhixiao Tu, and Huiling Zhu, and Haifeng Shi, and Shuang Li, and Yongqing Hou, and Chien-An Andy Hu
The role and regulation of MAFbx/atrogin-1 and MuRF1 in skeletal muscle atrophy.
March 2011, Pflugers Archiv : European journal of physiology,
Yulan Liu, and Xiuying Wang, and Weibo Leng, and Dingan Pi, and Zhixiao Tu, and Huiling Zhu, and Haifeng Shi, and Shuang Li, and Yongqing Hou, and Chien-An Andy Hu
Atrogin-1/MAFbx and MuRF1 are downregulated in aging-related loss of skeletal muscle.
July 2006, The journals of gerontology. Series A, Biological sciences and medical sciences,
Yulan Liu, and Xiuying Wang, and Weibo Leng, and Dingan Pi, and Zhixiao Tu, and Huiling Zhu, and Haifeng Shi, and Shuang Li, and Yongqing Hou, and Chien-An Andy Hu
Ghrelin receptor agonist GHRP-2 prevents arthritis-induced increase in E3 ubiquitin-ligating enzymes MuRF1 and MAFbx gene expression in skeletal muscle.
December 2005, American journal of physiology. Endocrinology and metabolism,
Copied contents to your clipboard!