Biomaterial Database

Monitoring complete and incomplete abscission in the germ line stem cell lineage of Drosophila ovaries. 2017

J Mathieu, and J-R Huynh

Institut Curie, Paris, France; CNRS UMR3215, Inserm U934, Paris, France.

In most species, cytokinesis is blocked in germ cells during at least some stage of their development. Abscission is difficult to assess directly in germ cells which are located in internal organs. Here, we described several indirect and direct methods to monitor the completion of abscission in Drosophila germ line cells. These methods are based on the observation that cells still connected by some cytoplasm share some degree of synchronization of their cell cycle. This synchrony can be detected on fixed tissue (Section 1.1), including using EdU incorporation to label S-phase (Section 1.2). Mitotic synchrony can also be observed using short-term live imaging (Section 1.3). Finally, we describe how the completion of abscission can be monitored using photoactivatable markers diffusing or not between two cells (Section 1.4).

UI	MeSH Term	Description	Entries
D010053	Ovary	The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.	Ovaries
D004331	Drosophila melanogaster	A species of fruit fly frequently used in genetics because of the large size of its chromosomes.	D. melanogaster,Drosophila melanogasters,melanogaster, Drosophila
D005260	Female		Females
D005854	Germ Cells	The reproductive cells in multicellular organisms at various stages during GAMETOGENESIS.	Gamete,Gametes,Germ-Line Cells,Germ Line,Cell, Germ,Cell, Germ-Line,Cells, Germ,Cells, Germ-Line,Germ Cell,Germ Line Cells,Germ Lines,Germ-Line Cell
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013234	Stem Cells	Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.	Colony-Forming Units,Mother Cells,Progenitor Cells,Colony-Forming Unit,Cell, Mother,Cell, Progenitor,Cell, Stem,Cells, Mother,Cells, Progenitor,Cells, Stem,Colony Forming Unit,Colony Forming Units,Mother Cell,Progenitor Cell,Stem Cell
D048749	Cytokinesis	The process by which the CYTOPLASM of a cell is divided.	Cytoplasmic Division,Cytokineses,Cytoplasmic Divisions,Division, Cytoplasmic,Divisions, Cytoplasmic
D057054	Molecular Imaging	The use of molecularly targeted imaging probes to localize and/or monitor biochemical and cellular processes via various imaging modalities that include RADIONUCLIDE IMAGING; ULTRASONOGRAPHY; MAGNETIC RESONANCE IMAGING; FLUORESCENCE IMAGING; and MICROSCOPY.	Imaging, Molecular
D058948	Cell Tracking	Non-invasive imaging of cells that have been labeled non-destructively, such as with nanoemulsions or reporter genes that can be detected by molecular imaging, to monitor their location, viability, cell lineage expansion, response to drugs, movement, or other behaviors in vivo.	Cell Trackings,Tracking, Cell,Trackings, Cell
D019070	Cell Lineage	The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.	Cell Lineages,Lineage, Cell,Lineages, Cell