BIOMAT Database Logo BIOMAT Database Logo

RAB10 Interacts with the Male Germ Cell-Specific GTPase-Activating Protein during Mammalian Spermiogenesis. 2017

Ying-Hung Lin, and Chih-Chun Ke, and Ya-Yun Wang, and Mei-Feng Chen, and Tsung-Ming Chen, and Wei-Chi Ku, and Han-Sun Chiang, and Chung-Hsin Yeh
Graduate Institute of Biomedical and Pharmaceutical Science, Fu-Jen Catholic University, New Taipei City 24205, Taiwan. 084952@mail.fju.edu.tw.

According to recent estimates, 2%-15% of couples are sterile, and approximately half of the infertility cases are attributed to male reproductive factors. However, the reasons remain undefined in approximately 25% of male infertility cases, and most infertility cases exhibit spermatogenic defects. Numerous genes involved in spermatogenesis still remain unknown. We previously identified Male Germ Cells Rab GTPase-Activating Proteins (MGCRABGAPs) through cDNA microarray analysis of human testicular tissues with spermatogenic defects. MGCRABGAP contains a conserved RABGAP catalytic domain, TBC (Tre2/Bub2/Cdc16). RABGAP family proteins regulate cellular function (e.g., cytoskeletal remodeling, vesicular trafficking, and cell migration) by inactivating RAB proteins. MGCRABGAP is a male germ cell-specific protein expressed in elongating and elongated spermatids during mammalian spermiogenesis. The purpose of this study was to identify proteins that interact with MGCRABGAP during mammalian spermiogenesis using a proteomic approach. We found that MGCRABGAP exhibited GTPase-activating bioability, and several MGCRABGAP interactors, possible substrates (e.g., RAB10, RAB5C, and RAP1), were identified using co-immunoprecipitation (co-IP) and nano liquid chromatography-mass spectrometry/mass spectrometry (nano LC-MS/MS). We confirmed the binding ability between RAB10 and MGCRABGAP via co-IP. Additionally, MGCRABGAP-RAB10 complexes were specifically colocalized in the manchette structure, a critical structure for the formation of spermatid heads, and were slightly expressed at the midpiece of mature spermatozoa. Based on these results, we propose that MGCRABGAP is involved in mammalian spermiogenesis by modulating RAB10.

UI MeSH Term Description Entries
D008297 Male Males
D008322 Mammals Warm-blooded vertebrate animals belonging to the class Mammalia, including all that possess hair and suckle their young. Mammalia,Mammal
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D002853 Chromatography, Liquid Chromatographic techniques in which the mobile phase is a liquid. Liquid Chromatography
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013077 Sperm Head The anterior portion of the spermatozoon (SPERMATOZOA) that contains mainly the nucleus with highly compact CHROMATIN material. Head, Sperm,Heads, Sperm,Sperm Heads
D013087 Spermatids Male germ cells derived from the haploid secondary SPERMATOCYTES. Without further division, spermatids undergo structural changes and give rise to SPERMATOZOA. Spermatoblasts,Spermatid,Spermatoblast
D013091 Spermatogenesis The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA. Spermatocytogenesis,Spermiogenesis
D013094 Spermatozoa Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility. Sperm,Spermatozoon,X-Bearing Sperm,X-Chromosome-Bearing Sperm,Y-Bearing Sperm,Y-Chromosome-Bearing Sperm,Sperm, X-Bearing,Sperm, X-Chromosome-Bearing,Sperm, Y-Bearing,Sperm, Y-Chromosome-Bearing,Sperms, X-Bearing,Sperms, X-Chromosome-Bearing,Sperms, Y-Bearing,Sperms, Y-Chromosome-Bearing,X Bearing Sperm,X Chromosome Bearing Sperm,X-Bearing Sperms,X-Chromosome-Bearing Sperms,Y Bearing Sperm,Y Chromosome Bearing Sperm,Y-Bearing Sperms,Y-Chromosome-Bearing Sperms

Related Publications

Ying-Hung Lin, and Chih-Chun Ke, and Ya-Yun Wang, and Mei-Feng Chen, and Tsung-Ming Chen, and Wei-Chi Ku, and Han-Sun Chiang, and Chung-Hsin Yeh
p115 Rho GTPase activating protein interacts with MEKK1.
August 2002, Journal of cellular physiology,
Ying-Hung Lin, and Chih-Chun Ke, and Ya-Yun Wang, and Mei-Feng Chen, and Tsung-Ming Chen, and Wei-Chi Ku, and Han-Sun Chiang, and Chung-Hsin Yeh
p120 Ras GTPase-activating protein interacts with Ras-GTP through specific conserved residues.
June 1996, The Journal of biological chemistry,
Ying-Hung Lin, and Chih-Chun Ke, and Ya-Yun Wang, and Mei-Feng Chen, and Tsung-Ming Chen, and Wei-Chi Ku, and Han-Sun Chiang, and Chung-Hsin Yeh
Evolutionary conservation of a germ cell-specific lamin persisting through mammalian spermiogenesis.
January 1992, Experimental cell research,
Ying-Hung Lin, and Chih-Chun Ke, and Ya-Yun Wang, and Mei-Feng Chen, and Tsung-Ming Chen, and Wei-Chi Ku, and Han-Sun Chiang, and Chung-Hsin Yeh
Mammalian male and female germ cells express a germ cell-specific Y-Box protein, MSY2.
November 1998, Biology of reproduction,
Ying-Hung Lin, and Chih-Chun Ke, and Ya-Yun Wang, and Mei-Feng Chen, and Tsung-Ming Chen, and Wei-Chi Ku, and Han-Sun Chiang, and Chung-Hsin Yeh
The cell surface during mammalian spermiogenesis.
March 1978, Developmental biology,
Ying-Hung Lin, and Chih-Chun Ke, and Ya-Yun Wang, and Mei-Feng Chen, and Tsung-Ming Chen, and Wei-Chi Ku, and Han-Sun Chiang, and Chung-Hsin Yeh
Protein-glycolipid interactions during spermatogenesis. Binding of specific germ cell proteins to sulfatoxygalactosylacylalkylglycerol, the major glycolipid of mammalian male germ cells.
October 1985, Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire,
Ying-Hung Lin, and Chih-Chun Ke, and Ya-Yun Wang, and Mei-Feng Chen, and Tsung-Ming Chen, and Wei-Chi Ku, and Han-Sun Chiang, and Chung-Hsin Yeh
A mammalian germ cell-specific RNA-binding protein interacts with ubiquitously expressed proteins involved in splice site selection.
May 2000, Proceedings of the National Academy of Sciences of the United States of America,
Ying-Hung Lin, and Chih-Chun Ke, and Ya-Yun Wang, and Mei-Feng Chen, and Tsung-Ming Chen, and Wei-Chi Ku, and Han-Sun Chiang, and Chung-Hsin Yeh
Murine germ cell-specific disruption of Ift172 causes defects in spermiogenesis and male fertility.
April 2020, Reproduction (Cambridge, England),
Ying-Hung Lin, and Chih-Chun Ke, and Ya-Yun Wang, and Mei-Feng Chen, and Tsung-Ming Chen, and Wei-Chi Ku, and Han-Sun Chiang, and Chung-Hsin Yeh
Rho family GTPase Rnd2 interacts and co-localizes with MgcRacGAP in male germ cells.
May 2003, The Biochemical journal,
Ying-Hung Lin, and Chih-Chun Ke, and Ya-Yun Wang, and Mei-Feng Chen, and Tsung-Ming Chen, and Wei-Chi Ku, and Han-Sun Chiang, and Chung-Hsin Yeh
Mammalian male germ cell cytogenetics.
March 1987, Mutagenesis,
Copied contents to your clipboard!