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With the intention of studying calcium-dependent ciliary reversal in Tetrahymena, we isolated a Tetrahymena calcium-binding protein of 10 kDa (TCBP-10) which was not calmodulin and reported its properties (Ohnishi, K., and Watanabe, Y. (1983) J. Biol. Chem. 258, 13978-13985). However, immunoblotting with an antiserum against TCBP-10 and sequencing of the cDNAs and partial genomic DNAs for this calcium-binding protein prove that this previously reported TCBP-10 is the degraded product of a 25-kDa calcium-binding protein. Thus, we correct the name of the protein from TCBP-10 to TCBP-25. From the analysis of the cDNA for TCBP-25, it is shown to be composed of 218 amino acid residues and its molecular weight is estimated to be 24,702. This protein is predicted to contain four EF-hand-type calcium binding domains and to be a member of the calmodulin family. Little sequence homology with other proteins was shown by a computer search, except in the EF-hand regions. The special feature of TCBP-25 is that the distance between calcium-binding domains II and III is extraordinarily long for a calmodulin family protein having four calcium-binding domains. The genomic DNA for TCBP-25 contains two introns situated at short distances before calcium-binding domains I and III, implying gene duplication in genealogy.
T Takemasa, and
K Ohnishi, and
T Kobayashi, and
T Takagi, and
K Konishi, and
Y Watanabe
January 2000,
Methods in cell biology,
T Takemasa, and
K Ohnishi, and
T Kobayashi, and
T Takagi, and
K Konishi, and
Y Watanabe
February 1990,
The Journal of biological chemistry,
T Takemasa, and
K Ohnishi, and
T Kobayashi, and
T Takagi, and
K Konishi, and
Y Watanabe
May 1996,
Journal of biochemistry,
T Takemasa, and
K Ohnishi, and
T Kobayashi, and
T Takagi, and
K Konishi, and
Y Watanabe
July 1988,
European journal of biochemistry,
T Takemasa, and
K Ohnishi, and
T Kobayashi, and
T Takagi, and
K Konishi, and
Y Watanabe
August 1995,
Experimental cell research,
T Takemasa, and
K Ohnishi, and
T Kobayashi, and
T Takagi, and
K Konishi, and
Y Watanabe
January 2000,
Journal of biochemistry,
T Takemasa, and
K Ohnishi, and
T Kobayashi, and
T Takagi, and
K Konishi, and
Y Watanabe
January 2008,
Cell structure and function,
T Takemasa, and
K Ohnishi, and
T Kobayashi, and
T Takagi, and
K Konishi, and
Y Watanabe
April 1992,
Biochemical and biophysical research communications,
T Takemasa, and
K Ohnishi, and
T Kobayashi, and
T Takagi, and
K Konishi, and
Y Watanabe
January 1986,
Biochimie,
T Takemasa, and
K Ohnishi, and
T Kobayashi, and
T Takagi, and
K Konishi, and
Y Watanabe
June 1992,
The Journal of biological chemistry,
