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It can be postulated that among the factors implicated in cartilaginous lesions, oxygen-derived free radicals seem to have a prominent part. To investigate this hypothesis, rabbit articular chondrocyte cultures have been exposed to oxygen-derived reactive species generated by the hypoxanthine-xanthine oxydase system. We observed a dose-dependent decrease of cellular growth. In order to explain this result, cell cycle progression and binucleate cell fractions have been studied. A greater number of binucleate cells and an increase in cell volume were observed. Flow cytometry analysis revealed a perturbation in cell cycle progression leading to a significant increase in the proportion of cells in G2 phase and an important augmentation in cell protein content confirmed by biochemical assays. This model shows which type of alteration can be induced by oxygen-derived free radicals in vitro. In addition, we deem this model to be useful for studying degenerative processes and for screening drugs that can scavenge oxygen-free radicals.
F Vincent, and
H Brun, and
E Clain, and
X Ronot, and
M Adolphe
January 1992,
Free radical research communications,
F Vincent, and
H Brun, and
E Clain, and
X Ronot, and
M Adolphe
February 1991,
Experimental cell research,
F Vincent, and
H Brun, and
E Clain, and
X Ronot, and
M Adolphe
September 1984,
Journal of cellular physiology,
F Vincent, and
H Brun, and
E Clain, and
X Ronot, and
M Adolphe
April 1984,
Annals of the rheumatic diseases,
F Vincent, and
H Brun, and
E Clain, and
X Ronot, and
M Adolphe
October 2012,
Nan fang yi ke da xue xue bao = Journal of Southern Medical University,
F Vincent, and
H Brun, and
E Clain, and
X Ronot, and
M Adolphe
June 1991,
Zhonghua bing li xue za zhi = Chinese journal of pathology,
F Vincent, and
H Brun, and
E Clain, and
X Ronot, and
M Adolphe
May 2000,
The Journal of rheumatology,
F Vincent, and
H Brun, and
E Clain, and
X Ronot, and
M Adolphe
October 1983,
Mechanisms of ageing and development,
F Vincent, and
H Brun, and
E Clain, and
X Ronot, and
M Adolphe
November 1983,
Cell and tissue kinetics,
F Vincent, and
H Brun, and
E Clain, and
X Ronot, and
M Adolphe
January 1983,
Annales pharmaceutiques francaises,
