The piggyBac transposon-derived genes TPB1 and TPB6 mediate essential transposon-like excision during the developmental rearrangement of key genes in Tetrahymena thermophila. 2016

Chao-Yin Cheng, and Janet M Young, and Chih-Yi Gabriela Lin, and Ju-Lan Chao, and Harmit S Malik, and Meng-Chao Yao
Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan.

Ciliated protozoans perform extreme forms of programmed somatic DNA rearrangement during development. The model ciliate Tetrahymena thermophila removes 34% of its germline micronuclear genome from somatic macronuclei by excising thousands of internal eliminated sequences (IESs), a process that shares features with transposon excision. Indeed, piggyBac transposon-derived genes are necessary for genome-wide IES excision in both Tetrahymena (TPB2 [Tetrahymena piggyBac-like 2] and LIA5) and Paramecium tetraurelia (PiggyMac). T. thermophila has at least three other piggyBac-derived genes: TPB1, TPB6, and TPB7 Here, we show that TPB1 and TPB6 excise a small, distinct set of 12 unusual IESs that disrupt exons. TPB1-deficient cells complete mating, but their progeny exhibit slow growth, giant vacuoles, and osmotic shock sensitivity due to retention of an IES in the vacuolar gene DOP1 (Dopey domain-containing protein). Unlike most IESs, TPB1-dependent IESs have piggyBac-like terminal inverted motifs that are necessary for excision. Transposon-like excision mediated by TPB1 and TPB6 provides direct evidence for a transposon origin of not only IES excision machinery but also IESs themselves. Our study highlights a division of labor among ciliate piggyBac-derived genes, which carry out mutually exclusive categories of excision events mediated by either transposon-like features or RNA-directed heterochromatin.

UI MeSH Term Description Entries
D008018 Life Cycle Stages The continuous sequence of changes undergone by living organisms during the post-embryonic developmental process, such as metamorphosis in insects and amphibians. This includes the developmental stages of apicomplexans such as the malarial parasite, PLASMODIUM FALCIPARUM. Life Cycle,Life History Stages,Cycle, Life,Cycles, Life,History Stage, Life,History Stages, Life,Life Cycle Stage,Life Cycles,Life History Stage,Stage, Life Cycle,Stage, Life History,Stages, Life Cycle,Stages, Life History
D004251 DNA Transposable Elements Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom. DNA Insertion Elements,DNA Transposons,IS Elements,Insertion Sequence Elements,Tn Elements,Transposable Elements,Elements, Insertion Sequence,Sequence Elements, Insertion,DNA Insertion Element,DNA Transposable Element,DNA Transposon,Element, DNA Insertion,Element, DNA Transposable,Element, IS,Element, Insertion Sequence,Element, Tn,Element, Transposable,Elements, DNA Insertion,Elements, DNA Transposable,Elements, IS,Elements, Tn,Elements, Transposable,IS Element,Insertion Element, DNA,Insertion Elements, DNA,Insertion Sequence Element,Sequence Element, Insertion,Tn Element,Transposable Element,Transposable Element, DNA,Transposable Elements, DNA,Transposon, DNA,Transposons, DNA
D014617 Vacuoles Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion. Vacuole
D015321 Gene Rearrangement The ordered rearrangement of gene regions by DNA recombination such as that which occurs normally during development. DNA Rearrangement,DNA Rearrangements,Gene Rearrangements,Rearrangement, DNA,Rearrangement, Gene,Rearrangements, DNA,Rearrangements, Gene
D015800 Protozoan Proteins Proteins found in any species of protozoan. Proteins, Protozoan
D016808 Tetrahymena thermophila A species of ciliate protozoa used in genetic and cytological research. Tetrahymena thermophilas,thermophilas, Tetrahymena
D017125 Genes, Protozoan The functional hereditary units of protozoa. Protozoan Genes,Gene, Protozoan,Protozoan Gene
D055786 Gene Knockout Techniques Techniques to alter a gene sequence that result in an inactivated gene, or one in which the expression can be inactivated at a chosen time during development to study the loss of function of a gene. Gene Knock-Out Techniques,Gene Knock Out,Gene Knock Out Techniques,Gene Knockout,Gene Knock Outs,Gene Knock-Out Technique,Gene Knockout Technique,Gene Knockouts,Knock Out, Gene,Knock Outs, Gene,Knock-Out Technique, Gene,Knock-Out Techniques, Gene,Knockout Technique, Gene,Knockout Techniques, Gene,Knockout, Gene,Knockouts, Gene,Out, Gene Knock,Outs, Gene Knock,Technique, Gene Knock-Out,Technique, Gene Knockout,Techniques, Gene Knock-Out,Techniques, Gene Knockout
D018503 Genome, Protozoan The complete genetic complement contained in a set of CHROMOSOMES in a protozoan. Protozoan Genome,Genomes, Protozoan,Protozoan Genomes
D018507 Gene Expression Regulation, Developmental Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism. Developmental Gene Expression Regulation,Embryologic Gene Expression Regulation,Gene Expression Regulation, Embryologic,Regulation of Gene Expression, Developmental,Regulation of Gene Expression, Embryologic,Regulation, Gene Expression, Developmental,Regulation, Gene Expression, Embryologic

Related Publications

Chao-Yin Cheng, and Janet M Young, and Chih-Yi Gabriela Lin, and Ju-Lan Chao, and Harmit S Malik, and Meng-Chao Yao
September 1997, Chromosoma,
Chao-Yin Cheng, and Janet M Young, and Chih-Yi Gabriela Lin, and Ju-Lan Chao, and Harmit S Malik, and Meng-Chao Yao
April 2016, Protein & cell,
Chao-Yin Cheng, and Janet M Young, and Chih-Yi Gabriela Lin, and Ju-Lan Chao, and Harmit S Malik, and Meng-Chao Yao
May 2010, Molecular biology of the cell,
Chao-Yin Cheng, and Janet M Young, and Chih-Yi Gabriela Lin, and Ju-Lan Chao, and Harmit S Malik, and Meng-Chao Yao
February 2014, The Plant journal : for cell and molecular biology,
Chao-Yin Cheng, and Janet M Young, and Chih-Yi Gabriela Lin, and Ju-Lan Chao, and Harmit S Malik, and Meng-Chao Yao
September 2017, Nucleic acids research,
Chao-Yin Cheng, and Janet M Young, and Chih-Yi Gabriela Lin, and Ju-Lan Chao, and Harmit S Malik, and Meng-Chao Yao
July 1982, Nucleic acids research,
Chao-Yin Cheng, and Janet M Young, and Chih-Yi Gabriela Lin, and Ju-Lan Chao, and Harmit S Malik, and Meng-Chao Yao
September 1991, Genetics,
Chao-Yin Cheng, and Janet M Young, and Chih-Yi Gabriela Lin, and Ju-Lan Chao, and Harmit S Malik, and Meng-Chao Yao
November 2018, Protein & cell,
Chao-Yin Cheng, and Janet M Young, and Chih-Yi Gabriela Lin, and Ju-Lan Chao, and Harmit S Malik, and Meng-Chao Yao
April 1985, Experimental cell research,
Chao-Yin Cheng, and Janet M Young, and Chih-Yi Gabriela Lin, and Ju-Lan Chao, and Harmit S Malik, and Meng-Chao Yao
January 2012, Biochemical and biophysical research communications,
Copied contents to your clipboard!