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This study, using immunocytochemical light and electron microscopy techniques, characterizes the distribution of three antibodies bound to the surface of rat glomerular visceral epithelial cells (GEC) in culture, and tests their ability to redistribute corresponding antigens under conditions appropriate for antigenic modulation (antigen disappearance). At 4 degrees C or after fixation, anti-renal tubular brush border vesicle (BBV) IgG bound diffusely to the surface of GEC and to coated pits. Anti-gp330 IgG had a discrete distribution on the surface of GEC and reacted with coated pits. Anti-podocalyxin IgG was bound diffusely to the surface of GEC but not to coated pits. At 37 degrees C, anti-BBV IgG induced marked redistribution of immune complexes with both shedding and internalization. Anti-gp330 IgG induced weaker redistribution, with internalization of immune complexes predominating. Anti-podocalyxin IgG induced rapid redistribution of immune complexes and antigenic modulation but minimal internalization. Experiments of differential redistribution indicated that anti-BBV IgG modulated the expression of both gp330 and podocalyxin; anti-gp330 IgG had a weaker effect on BBV antigens and podocalyxin; and anti-podocalyxin failed to redistribute BBV antigens or gp330. The relevance of these immunocytochemical studies of antibody-cell surface antigen interaction in cultured GEC to understanding the pathogenesis of Heymann glomerulonephritis (HG) is discussed.
G Camussi, and
D Kerjaschki, and
M Gonda, and
T Nevins, and
J C Rielle, and
J Brentjens, and
G Andres
February 1995,
Journal of the American Society of Nephrology : JASN,
G Camussi, and
D Kerjaschki, and
M Gonda, and
T Nevins, and
J C Rielle, and
J Brentjens, and
G Andres
February 1989,
British journal of experimental pathology,
G Camussi, and
D Kerjaschki, and
M Gonda, and
T Nevins, and
J C Rielle, and
J Brentjens, and
G Andres
June 1995,
European journal of cell biology,
G Camussi, and
D Kerjaschki, and
M Gonda, and
T Nevins, and
J C Rielle, and
J Brentjens, and
G Andres
November 1987,
The American journal of pathology,
G Camussi, and
D Kerjaschki, and
M Gonda, and
T Nevins, and
J C Rielle, and
J Brentjens, and
G Andres
September 1987,
Laboratory investigation; a journal of technical methods and pathology,
G Camussi, and
D Kerjaschki, and
M Gonda, and
T Nevins, and
J C Rielle, and
J Brentjens, and
G Andres
December 1994,
Cellular and molecular biology (Noisy-le-Grand, France),
G Camussi, and
D Kerjaschki, and
M Gonda, and
T Nevins, and
J C Rielle, and
J Brentjens, and
G Andres
January 1990,
Northwestern dental research,
G Camussi, and
D Kerjaschki, and
M Gonda, and
T Nevins, and
J C Rielle, and
J Brentjens, and
G Andres
January 1993,
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,
G Camussi, and
D Kerjaschki, and
M Gonda, and
T Nevins, and
J C Rielle, and
J Brentjens, and
G Andres
April 2001,
Journal of the American Society of Nephrology : JASN,
G Camussi, and
D Kerjaschki, and
M Gonda, and
T Nevins, and
J C Rielle, and
J Brentjens, and
G Andres
June 1971,
Annals of the New York Academy of Sciences,
