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Equine herpesvirus type 1 (EHV-1) causes respiratory infections, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single-point mutation in DNA polymerase gene, resulting in an amino acid variation (N752/D752), is significantly associated with the neuropathogenic potential of EHV-1 strains. The aim of the study was to elucidate if there are any differences between neuropathogenic (EHV-1 26) and non-neuropathogenic (Jan-E and Rac-H) EHV-1 strains in their ability to infect neuronal cells. For the tested EHV-1 strains, cytopathic effect (CPE) was manifested by changed morphology of cells, destruction of actin cytoskeleton and nuclei degeneration, which led to focal degeneration. Moreover, EHV-1 26 strain caused fusion of the infected cells to form syncytia in culture. Real-time PCR analysis demonstrated that both neuropathogenic and non-neuropathogenic EHV-1 strains replicated in neurons and ED cells (equine dermal cell line) at a similar level. We can assume that a point mutation in the EHV-1 polymerase does not affect viral replication in this cell type.
J Cymerys, and
A Słońska, and
J Brzezicka, and
A Tucholska, and
A Chmielewska, and
J Rola, and
P Malik, and
M W Bańbura
January 2013,
Polish journal of veterinary sciences,
J Cymerys, and
A Słońska, and
J Brzezicka, and
A Tucholska, and
A Chmielewska, and
J Rola, and
P Malik, and
M W Bańbura
January 1978,
IARC scientific publications,
J Cymerys, and
A Słońska, and
J Brzezicka, and
A Tucholska, and
A Chmielewska, and
J Rola, and
P Malik, and
M W Bańbura
August 2010,
The Journal of general virology,
J Cymerys, and
A Słońska, and
J Brzezicka, and
A Tucholska, and
A Chmielewska, and
J Rola, and
P Malik, and
M W Bańbura
October 2010,
Veterinary microbiology,
J Cymerys, and
A Słońska, and
J Brzezicka, and
A Tucholska, and
A Chmielewska, and
J Rola, and
P Malik, and
M W Bańbura
February 2010,
Veterinary microbiology,
J Cymerys, and
A Słońska, and
J Brzezicka, and
A Tucholska, and
A Chmielewska, and
J Rola, and
P Malik, and
M W Bańbura
February 2017,
Comparative immunology, microbiology and infectious diseases,
J Cymerys, and
A Słońska, and
J Brzezicka, and
A Tucholska, and
A Chmielewska, and
J Rola, and
P Malik, and
M W Bańbura
November 2010,
Veterinary journal (London, England : 1997),
J Cymerys, and
A Słońska, and
J Brzezicka, and
A Tucholska, and
A Chmielewska, and
J Rola, and
P Malik, and
M W Bańbura
January 2011,
Veterinary microbiology,
J Cymerys, and
A Słońska, and
J Brzezicka, and
A Tucholska, and
A Chmielewska, and
J Rola, and
P Malik, and
M W Bańbura
November 2009,
Veterinary microbiology,
J Cymerys, and
A Słońska, and
J Brzezicka, and
A Tucholska, and
A Chmielewska, and
J Rola, and
P Malik, and
M W Bańbura
January 1986,
New Zealand veterinary journal,
