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Freeze-substitution as a preparation technique for frozen specimens is briefly reviewed. Recent advance in cryotechniques, especially quick freezing followed by freeze-substitution, has made it possible to preserve the ultrastructural features of cells and tissues to reflect more closely their living state and capture transient physiological processes as evanescent as fusion and fission of biological membranes. The technique is also advantageous to retain soluble substances of the cellular components. Thus, histochemical, autoradiographic and/or immunocytochemical analyses of quick-frozen, substitution-fixed samples allow us to obtain more precise localization of chemical components in cells than in the case of conventional chemical fixation. Several examples indicating the superiority of the preparatory method are presented.
A Ichikawa, and
M Ichikawa, and
K Sasaki
January 1994,
Scanning microscopy. Supplement,
A Ichikawa, and
M Ichikawa, and
K Sasaki
February 2008,
Biology of the cell,
A Ichikawa, and
M Ichikawa, and
K Sasaki
January 1986,
Scanning electron microscopy,
A Ichikawa, and
M Ichikawa, and
K Sasaki
January 1996,
Acta cytologica,
A Ichikawa, and
M Ichikawa, and
K Sasaki
January 1977,
Current topics in pathology. Ergebnisse der Pathologie,
A Ichikawa, and
M Ichikawa, and
K Sasaki
August 1975,
Journal of microscopy,
A Ichikawa, and
M Ichikawa, and
K Sasaki
June 1994,
Microscopy research and technique,
A Ichikawa, and
M Ichikawa, and
K Sasaki
January 1983,
Scanning electron microscopy,
A Ichikawa, and
M Ichikawa, and
K Sasaki
July 1968,
Medical & biological illustration,
A Ichikawa, and
M Ichikawa, and
K Sasaki
April 1993,
Microscopy research and technique,
