Biomaterial Database

Expert system computer program for troubleshooting the gel-clot Limulus amebocyte lysate (LAL) assay. 1989

K Tsuji

This article describes a knowledge-based or expert system computer program that can assist in troubleshooting the gel-clot Limulus Amebocyte Lysate (LAL) method for the assay of endotoxin in parenteral products and in-process materials. The program may also be used to train new personnel and/or to assist in the development of an LAL method for a new product or material. The program includes LAL assay information for 31 parenteral products and materials. The program uses rules-of-thumb and intuition to diagnose 155 possible LAL assay problems and logically reduce them to 37 recommended problem solving processes. The program also provides references to support its recommendation.

UI	MeSH Term	Description	Entries
D008033	Limulus Test	Sensitive method for detection of bacterial endotoxins and endotoxin-like substances that depends on the in vitro gelation of Limulus amebocyte lysate (LAL), prepared from the circulating blood (amebocytes) of the horseshoe crab, by the endotoxin or related compound. Used for detection of endotoxin in body fluids and parenteral pharmaceuticals.	Limulus Tests,Test, Limulus,Tests, Limulus
D004731	Endotoxins	Toxins closely associated with the living cytoplasm or cell wall of certain microorganisms, which do not readily diffuse into the culture medium, but are released upon lysis of the cells.	Endotoxin
D005103	Expert Systems	Computer programs based on knowledge developed from consultation with experts on a problem, and the processing and/or formalizing of this knowledge using these programs in such a manner that the problems may be solved.	Expert System,System, Expert,Systems, Expert
D012984	Software	Sequential operating programs and data which instruct the functioning of a digital computer.	Computer Programs,Computer Software,Open Source Software,Software Engineering,Software Tools,Computer Applications Software,Computer Programs and Programming,Computer Software Applications,Application, Computer Software,Applications Software, Computer,Applications Softwares, Computer,Applications, Computer Software,Computer Applications Softwares,Computer Program,Computer Software Application,Engineering, Software,Open Source Softwares,Program, Computer,Programs, Computer,Software Application, Computer,Software Applications, Computer,Software Tool,Software, Computer,Software, Computer Applications,Software, Open Source,Softwares, Computer Applications,Softwares, Open Source,Source Software, Open,Source Softwares, Open,Tool, Software,Tools, Software