-transparent.png){kind=logo}
Cyclic-nucleotide-gated channels are essential for vision and olfaction. They belong to the voltage-gated ion channel superfamily but their activities are controlled by intracellular cyclic nucleotides instead of transmembrane voltage. Here we report a 3.5-Å-resolution single-particle electron cryo-microscopy structure of a cyclic-nucleotide-gated channel from Caenorhabditis elegans in the cyclic guanosine monophosphate (cGMP)-bound open state. The channel has an unusual voltage-sensor-like domain, accounting for its deficient voltage dependence. A carboxy-terminal linker connecting S6 and the cyclic-nucleotide-binding domain interacts directly with both the voltage-sensor-like domain and the pore domain, forming a gating ring that couples conformational changes triggered by cyclic nucleotide binding to the gate. The selectivity filter is lined by the carboxylate side chains of a functionally important glutamate and three rings of backbone carbonyls. This structure provides a new framework for understanding mechanisms of ion permeation, gating and channelopathy of cyclic-nucleotide-gated channels and cyclic nucleotide modulation of related channels.
Minghui Li, and
Xiaoyuan Zhou, and
Shu Wang, and
Ioannis Michailidis, and
Ye Gong, and
Deyuan Su, and
Huan Li, and
Xueming Li, and
Jian Yang
April 2017,
Proceedings of the National Academy of Sciences of the United States of America,
Minghui Li, and
Xiaoyuan Zhou, and
Shu Wang, and
Ioannis Michailidis, and
Ye Gong, and
Deyuan Su, and
Huan Li, and
Xueming Li, and
Jian Yang
January 2022,
Nature structural & molecular biology,
Minghui Li, and
Xiaoyuan Zhou, and
Shu Wang, and
Ioannis Michailidis, and
Ye Gong, and
Deyuan Su, and
Huan Li, and
Xueming Li, and
Jian Yang
December 2014,
PLoS computational biology,
Minghui Li, and
Xiaoyuan Zhou, and
Shu Wang, and
Ioannis Michailidis, and
Ye Gong, and
Deyuan Su, and
Huan Li, and
Xueming Li, and
Jian Yang
July 2005,
Journal of molecular biology,
Minghui Li, and
Xiaoyuan Zhou, and
Shu Wang, and
Ioannis Michailidis, and
Ye Gong, and
Deyuan Su, and
Huan Li, and
Xueming Li, and
Jian Yang
April 2023,
Nature structural & molecular biology,
Minghui Li, and
Xiaoyuan Zhou, and
Shu Wang, and
Ioannis Michailidis, and
Ye Gong, and
Deyuan Su, and
Huan Li, and
Xueming Li, and
Jian Yang
November 1994,
Nature,
Minghui Li, and
Xiaoyuan Zhou, and
Shu Wang, and
Ioannis Michailidis, and
Ye Gong, and
Deyuan Su, and
Huan Li, and
Xueming Li, and
Jian Yang
August 1993,
FEBS letters,
Minghui Li, and
Xiaoyuan Zhou, and
Shu Wang, and
Ioannis Michailidis, and
Ye Gong, and
Deyuan Su, and
Huan Li, and
Xueming Li, and
Jian Yang
March 2007,
Biochemical and biophysical research communications,
Minghui Li, and
Xiaoyuan Zhou, and
Shu Wang, and
Ioannis Michailidis, and
Ye Gong, and
Deyuan Su, and
Huan Li, and
Xueming Li, and
Jian Yang
August 2001,
Nature,
Minghui Li, and
Xiaoyuan Zhou, and
Shu Wang, and
Ioannis Michailidis, and
Ye Gong, and
Deyuan Su, and
Huan Li, and
Xueming Li, and
Jian Yang
November 2022,
International journal of molecular sciences,
