Molecular and Quantum Mechanical Study for the Separation of Cefprozil in the Presence of Its Alkaline Degradation Product Using RP-HPLC with UV Detection. 2017

Khalid A M Attia, and Mohammed W I Nassar, and Mohamed B El-Zeiny, and Ahmed Serag
Al-Azhar University, Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, 11751 Nasr City, Cairo, Egypt.

A reversed-phase HPLC method (RP-HPLC) with UV detection was developed and validated for the quantitative determination of cefprozil, a second-generation cephalosporin. Due to β-lactam ring instability under alkaline conditions, this RP-HPLC method was applied for the determination of cefprozil in the presence of its possible degradation product. The interactions that govern the separation process with stationary phase were investigated at both molecular and quantum mechanical levels. Moreover, electrostatic potential maps were generated to determine the sites of interaction with mobile phase. The suggested method was validated in compliance with International Conference on Harmonization guidelines and successfully applied for the determination of cefprozil in its commercial pharmaceutical formulation.

UI MeSH Term Description Entries
D008956 Models, Chemical Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment. Chemical Models,Chemical Model,Model, Chemical
D010879 Piperazines Compounds that are derived from PIPERAZINE.
D011789 Quantum Theory The theory that the radiation and absorption of energy take place in definite quantities called quanta (E) which vary in size and are defined by the equation E Quantum Theories,Theories, Quantum,Theory, Quantum
D002511 Cephalosporins A group of broad-spectrum antibiotics first isolated from the Mediterranean fungus ACREMONIUM. They contain the beta-lactam moiety thia-azabicyclo-octenecarboxylic acid also called 7-aminocephalosporanic acid. Antibiotics, Cephalosporin,Cephalosporanic Acid,Cephalosporin,Cephalosporin Antibiotic,Cephalosporanic Acids,Acid, Cephalosporanic,Acids, Cephalosporanic,Antibiotic, Cephalosporin,Cephalosporin Antibiotics
D000097670 Cefprozil A second-generation cephalosporin antibacterial agent. Cefprozil has a phenyl ring at the C-3 position of its cephalosporin core. Cefprozil Monohydrate,Cefprozil Sulfate,Arzimol,BMY 28100,BMY-28100,Brisoral,Cefzil,Procef,28100, BMY,Monohydrate, Cefprozil,Sulfate, Cefprozil
D012972 Sodium Hydroxide A highly caustic substance that is used to neutralize acids and make sodium salts. (From Merck Index, 11th ed) Caustic Soda,Hydroxide, Sodium,Soda, Caustic
D013056 Spectrophotometry, Ultraviolet Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Ultraviolet Spectrophotometry
D056148 Chromatography, Reverse-Phase A chromatography technique in which the stationary phase is composed of a non-polar substance with a polar mobile phase, in contrast to normal-phase chromatography in which the stationary phase is a polar substance with a non-polar mobile phase. Chromatography, Reversed-Phase Liquid,Reversed-Phase Chromatography,Reversed-Phase Liquid Chromatography,Reverse-Phase Chromatography,Reverse-Phase Liquid Chromatography,Chromatography, Reverse Phase,Chromatography, Reversed-Phase,Reverse Phase Chromatography,Reversed Phase Chromatography
D057927 Hydrophobic and Hydrophilic Interactions The thermodynamic interaction between a substance and WATER. Hydrophilic Interactions,Hydrophilic and Hydrophobic Interactions,Hydrophilicity,Hydrophobic Interactions,Hydrophobicity,Hydrophilic Interaction,Hydrophilicities,Hydrophobic Interaction,Hydrophobicities,Interaction, Hydrophilic,Interaction, Hydrophobic,Interactions, Hydrophilic,Interactions, Hydrophobic

Related Publications

Khalid A M Attia, and Mohammed W I Nassar, and Mohamed B El-Zeiny, and Ahmed Serag
October 2012, Biomedical chromatography : BMC,
Khalid A M Attia, and Mohammed W I Nassar, and Mohamed B El-Zeiny, and Ahmed Serag
February 2010, Journal of chromatographic science,
Khalid A M Attia, and Mohammed W I Nassar, and Mohamed B El-Zeiny, and Ahmed Serag
October 2014, Journal of chromatographic science,
Khalid A M Attia, and Mohammed W I Nassar, and Mohamed B El-Zeiny, and Ahmed Serag
August 2010, Journal of chromatographic science,
Khalid A M Attia, and Mohammed W I Nassar, and Mohamed B El-Zeiny, and Ahmed Serag
June 1994, Farmaco (Societa chimica italiana : 1989),
Khalid A M Attia, and Mohammed W I Nassar, and Mohamed B El-Zeiny, and Ahmed Serag
October 2005, Pakistan journal of pharmaceutical sciences,
Khalid A M Attia, and Mohammed W I Nassar, and Mohamed B El-Zeiny, and Ahmed Serag
March 2000, Se pu = Chinese journal of chromatography,
Khalid A M Attia, and Mohammed W I Nassar, and Mohamed B El-Zeiny, and Ahmed Serag
April 2003, Journal of pharmaceutical and biomedical analysis,
Khalid A M Attia, and Mohammed W I Nassar, and Mohamed B El-Zeiny, and Ahmed Serag
March 2016, Journal of the science of food and agriculture,
Copied contents to your clipboard!