Evaluation of TA10 Broth for Recovery of Listeria monocytogenes from Ground Beef. 2017

Naoko Kamisaki-Horikoshi, and Yukio Okada, and Kazuko Takeshita, and Makoto Takada, and Shinichi Kawamoto, and Susumu Kawasaki
PRIMA Meat Packers Ltd, 635 Nakamukaihara, Tsuchiura, Ibaraki 300-0841, Japan.

In 2009, the enrichment broth TA10 was released for simultaneous recovery of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7. This medium was compared with other Salmonella enrichment broths [lactose (LAC) broth, buffered peptone water (BPW), and universal pre-enrichment (UP) broth] for the recovery of heat- and freeze-injured Salmonella spp. in beef by the conventional culture method. There was a significant difference between TA10 and LAC enrichment broths for detecting injured Salmonella spp. In this study, the International Organization for Standardization Listeria pre-enrichment broth (Half-Fraser/Fraser) was compared with TA10 broth for the recovery of L. monocytogenes from ground beef. Ground beef samples were contaminated with single Listeria serovars at levels of 0.096 to 0.001 most probable number/g. Twenty 25 g test portions of the contaminated ground beef were pre-enriched in each broth, and the ISO-11290-1 Listeria official isolation protocol was used thereafter. There was a significant difference between TA10 broth (48 h) and Half-Fraser/Fraser broth (72 h) in the recovery of L. monocytogenes. In addition, the incubation time for TA10 broth was shorter than for Half-Fraser/Fraser broth. The results indicate that TA10 broth should be used instead of Half-Fraser/Fraser broth for analysis of beef that may be contaminated with very low levels of L. monocytogenes.

UI MeSH Term Description Entries
D008089 Listeria monocytogenes A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D000069466 Red Meat Meat such as beef, goat, pork, or lamb which contains more MYOGLOBIN than POULTRY or SEAFOOD. Lamb Meat,Veal,Beef,Lamb Meats,Meat, Lamb,Meat, Red,Meats, Lamb,Meats, Red,Red Meats
D016009 Chi-Square Distribution A distribution in which a variable is distributed like the sum of the squares of any given independent random variable, each of which has a normal distribution with mean of zero and variance of one. The chi-square test is a statistical test based on comparison of a test statistic to a chi-square distribution. The oldest of these tests are used to detect whether two or more population distributions differ from one another. Chi-Square Test,Chi Square Distribution,Chi Square Test,Chi-Square Distributions,Chi-Square Tests,Distribution, Chi-Square,Distributions, Chi-Square,Test, Chi-Square,Tests, Chi-Square

Related Publications

Naoko Kamisaki-Horikoshi, and Yukio Okada, and Kazuko Takeshita, and Makoto Takada, and Shinichi Kawamoto, and Susumu Kawasaki
May 1988, International journal of food microbiology,
Naoko Kamisaki-Horikoshi, and Yukio Okada, and Kazuko Takeshita, and Makoto Takada, and Shinichi Kawamoto, and Susumu Kawasaki
May 2011, Wei sheng wu xue bao = Acta microbiologica Sinica,
Naoko Kamisaki-Horikoshi, and Yukio Okada, and Kazuko Takeshita, and Makoto Takada, and Shinichi Kawamoto, and Susumu Kawasaki
August 1988, Journal of food protection,
Naoko Kamisaki-Horikoshi, and Yukio Okada, and Kazuko Takeshita, and Makoto Takada, and Shinichi Kawamoto, and Susumu Kawasaki
April 2015, Food microbiology,
Naoko Kamisaki-Horikoshi, and Yukio Okada, and Kazuko Takeshita, and Makoto Takada, and Shinichi Kawamoto, and Susumu Kawasaki
April 1991, Journal of food protection,
Naoko Kamisaki-Horikoshi, and Yukio Okada, and Kazuko Takeshita, and Makoto Takada, and Shinichi Kawamoto, and Susumu Kawasaki
September 1997, Journal of food protection,
Naoko Kamisaki-Horikoshi, and Yukio Okada, and Kazuko Takeshita, and Makoto Takada, and Shinichi Kawamoto, and Susumu Kawasaki
January 2012, Revista Argentina de microbiologia,
Naoko Kamisaki-Horikoshi, and Yukio Okada, and Kazuko Takeshita, and Makoto Takada, and Shinichi Kawamoto, and Susumu Kawasaki
November 1988, Applied and environmental microbiology,
Naoko Kamisaki-Horikoshi, and Yukio Okada, and Kazuko Takeshita, and Makoto Takada, and Shinichi Kawamoto, and Susumu Kawasaki
March 2019, International journal of food microbiology,
Naoko Kamisaki-Horikoshi, and Yukio Okada, and Kazuko Takeshita, and Makoto Takada, and Shinichi Kawamoto, and Susumu Kawasaki
May 1993, International journal of food microbiology,
Copied contents to your clipboard!