BIOMAT Database Logo BIOMAT Database Logo

[Secretion of pectate lyase and protein composition of the outer membrane of Erwinia chrysanthemi ENA49 cells]. 1989

V E Shevchik, and A N Evtushenkov, and Iu K Fomichev

The combined changes (specters of isoenzymes of intracellular and extracellular pectatelyase, protein composition of periplasm and outer membrane) in the cells of E. chrysanthemi ENA49 from the periodical culture growing on the inducer containing medium have been studied. The beginning of active pectatelyase synthesis was established to be accompanied by the temporal intracellular accumulation of the enzyme. The cellular capability of pectatelyase secretion to the outer medium correlates with the presence of the definite protein in the outer membrane. The different pathways for secretion of pectatelyase isoenzymes PLb, PLc, PLd and PLe are suggested.

UI MeSH Term Description Entries
D007527 Isoenzymes Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics. Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D011133 Polysaccharide-Lyases A group of carbon-oxygen lyases. These enzymes catalyze the breakage of a carbon-oxygen bond in polysaccharides leading to an unsaturated product and the elimination of an alcohol. EC 4.2.2. Polysaccharide Lyase,Polysaccharide-Lyase,Lyase, Polysaccharide,Polysaccharide Lyases
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004885 Erwinia A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria whose organisms are associated with plants as pathogens, saprophytes, or as constituents of the epiphytic flora.
D001425 Bacterial Outer Membrane Proteins Proteins isolated from the outer membrane of Gram-negative bacteria. OMP Proteins,Outer Membrane Proteins, Bacterial,Outer Membrane Lipoproteins, Bacterial

Related Publications

V E Shevchik, and A N Evtushenkov, and Iu K Fomichev
Cloning and analysis of structural and regulatory pectate lyase genes of Erwinia chrysanthemi ENA49.
September 1989, Gene,
V E Shevchik, and A N Evtushenkov, and Iu K Fomichev
Pectate lyase gene regulatory mutants of Erwinia chrysanthemi.
July 1986, Journal of bacteriology,
V E Shevchik, and A N Evtushenkov, and Iu K Fomichev
[recA-genes of Erwinia chrysanthemi ENA49].
January 1989, Molekuliarnaia genetika, mikrobiologiia i virusologiia,
V E Shevchik, and A N Evtushenkov, and Iu K Fomichev
The crystal structure of pectate lyase Pel9A from Erwinia chrysanthemi.
March 2004, The Journal of biological chemistry,
V E Shevchik, and A N Evtushenkov, and Iu K Fomichev
Molecular cloning of the outJ gene involved in pectate lyase secretion by Erwinia chrysanthemi.
March 1989, Molecular microbiology,
V E Shevchik, and A N Evtushenkov, and Iu K Fomichev
Organization of a pectate lyase gene family in Erwinia chrysanthemi.
January 1986, Gene,
V E Shevchik, and A N Evtushenkov, and Iu K Fomichev
Biochemical characterization of the pectate lyase PelZ of Erwinia chrysanthemi 3937.
April 1998, Biochimica et biophysica acta,
V E Shevchik, and A N Evtushenkov, and Iu K Fomichev
In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi.
March 1985, The EMBO journal,
V E Shevchik, and A N Evtushenkov, and Iu K Fomichev
Extracellular secretion of pectate lyase by the Erwinia chrysanthemi out pathway is dependent upon Sec-mediated export across the inner membrane.
July 1991, Journal of bacteriology,
V E Shevchik, and A N Evtushenkov, and Iu K Fomichev
Pectate lyase PelI of Erwinia chrysanthemi 3937 belongs to a new family.
December 1997, Journal of bacteriology,
Copied contents to your clipboard!